دورية أكاديمية
Identification of a Sesquiterpene Lactone from Arctium lappa Leaves with Antioxidant Activity in Primary Human Muscle Cells
| العنوان: | Identification of a Sesquiterpene Lactone from Arctium lappa Leaves with Antioxidant Activity in Primary Human Muscle Cells |
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| المؤلفون: | El Khatib, Nour, Morel, Sylvie, Hugon, Gerald, Rapior, Sylvie, Carnac, Gilles, Saint, Nathalie |
| المساهمون: | Physiologie & médecine expérimentale du Cœur et des Muscles U 1046 (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), This work was supported by CNRS, INSERM, and by Montpellier UniversityGrants. The authors are grateful to Aurélien Lebrun from the Laboratoire des Mesures Physiques(University of Montpellier, France) for NMR data acquisition, to Marie Jerzewski from the collectivegarden “Le Jardin Des Aubes” of Montpellier (France) where Arctium lappa leaves were collected andto Jean-Marc Donnay for technical assistance to collect Arctium lappa leaves. |
| المصدر: | ISSN: 1420-3049 ; Molecules ; https://hal.umontpellier.fr/hal-03157239Test ; Molecules, 2021, 26 (5), pp.1328. ⟨10.3390/molecules26051328⟩. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Arctium lappa L, Burdock leaves, Antioxidant, Onopordopicrin, Human myoblasts, MESH: Antioxidants, MESH: Arctium, MESH: Humans, MESH: Hydrogen Peroxide, MESH: Lactones, MESH: Muscle, Skeletal, MESH: Phytochemicals, MESH: Plant Extracts, MESH: Plant Leaves, MESH: Sesquiterpenes, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics |
| الوصف: | International audience ; Many pathologies affecting muscles (muscular dystrophies, sarcopenia, cachexia, renal insufficiency, obesity, diabetes type 2, etc.) are now clearly linked to mechanisms involving oxidative stress. In this context, there is a growing interest in exploring plants to find new natural antioxidants toprevent the appearance and the development of these muscle disorders. In this study, we investigated the antioxidant properties of Arctium lappa leaves in a model of primary human muscle cells exposed to H2O2 oxidative stress. We identified using bioassay-guided purification, onopordopicrin, a sesquiterpene lactone as the main molecule responsible for the antioxidant activity of A. lappa leaf extract. According to our findings, onopordopicrin inhibited the H2O2-mediated loss of muscle cell viability, by limiting the production of free radicals and abolishing DNA cellular damages. Moreover,we showed that onopordopicrin promoted the expression of the nuclear factor-erythroid-2-related factor 2 (Nrf2) downstream target protein heme oxygenase-1 (HO-1) in muscle cells. By using siRNA, we demonstrated that the inhibition of the expression of Nrf2 reduced the protective effect ofonopordopicrin, indicating that the activation of the Nrf2/HO-1 signaling pathway mediates the antioxidant effect of onopordopicrin in primary human muscle cells. Therefore, our results suggest that onopordopicrin may be a potential therapeutic molecule to fight against oxidative stress inpathological specific muscle disorder. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/33801315; hal-03157239; https://hal.umontpellier.fr/hal-03157239Test; https://hal.umontpellier.fr/hal-03157239v2/documentTest; https://hal.umontpellier.fr/hal-03157239v2/file/molecules-26-01328.pdfTest; PUBMED: 33801315; PUBMEDCENTRAL: PMC7958318; WOS: 000628439000001 |
| DOI: | 10.3390/molecules26051328 |
| الإتاحة: | https://doi.org/10.3390/molecules26051328Test https://hal.umontpellier.fr/hal-03157239Test https://hal.umontpellier.fr/hal-03157239v2/documentTest https://hal.umontpellier.fr/hal-03157239v2/file/molecules-26-01328.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.9AFCBC07 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/molecules26051328 |
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