دورية أكاديمية
Activity of Epsilon-poly-L-lysine against Multidrug-Resistant Pseudomonas aeruginosa and Klebsiella pneumoniae Isolates of Urinary Tract Infections
| العنوان: | Activity of Epsilon-poly-L-lysine against Multidrug-Resistant Pseudomonas aeruginosa and Klebsiella pneumoniae Isolates of Urinary Tract Infections |
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| المؤلفون: | de Sousa, Telma, Sabença, Carolina, Ribeiro, Miguel, Pino-Hurtado, Mario, Torres, Carmen, Hébraud, Michel, Alves, Olimpia, Sousa, Sara, Costa, Eliana, Igrejas, Gilberto, Poeta, Patrícia |
| المساهمون: | MicroArt, Universidade de Trás-os-Montes e Alto Douro (UTAD), Universidade do Trás-os-Montes e Alto Douro, Functional Genomics and Proteomics Unit, University of Trás-os-Montes and Alto Douro (Portugal), State University of Campinas, Universidad de La Rioja (UR), Department of Agriculture and Food, University of La Rioja, Microbiologie Environnement Digestif Santé (MEDIS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), hospital centre of Tras-os-Montes and Alto Douro |
| المصدر: | ISSN: 2227-9059 ; Biomedicines ; https://hal.inrae.fr/hal-04520242Test ; Biomedicines, 2024, 12 (3), pp.638. ⟨10.3390/biomedicines12030638⟩. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2024 |
| المجموعة: | HAL Clermont Auvergne (Université Blaise Pascal Clermont-Ferrand / Université d'Auvergne) |
| مصطلحات موضوعية: | Pseudomonas aeruginosa, Klebsiella pneumoniae, antibiotic resistance, biofilm, Epsilon-poly-L-lysine, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Pseudomonas aeruginosa and Klebsiella pneumoniae are notorious for their resistance to antibiotics and propensity for biofilm formation, posing significant threats to human health. Epsilon-poly-L-lysine (ε-PL) emerges as a naturally occurring antimicrobial poly(amino acid), which positions it as a prospective agent for addressing challenges linked to multidrug resistance. ε-PL symbolizes a promising avenue in the pursuit of efficacious therapeutic strategies and warrants earnest consideration within the realm of clinical treatment. Thus, our objective was to determine the antibiotic susceptibility profiles of 38 selected P. aeruginosa and ESBL-producing K. pneumoniae clinical isolates and determine the ability of ε-PL to inhibit biofilm formation. After PCR analysis, detection of genes related to β-lactamases was observed among the selected isolates of P. aeruginosa [blaSPM (35.7%), blaKPC (35.7%), blaSHV (14.3%), blaCTX-M (14.3%), blaOXA (14.3%), blaTEM (7.1%), blaPER (7.1%), blaVIM (7.1%), and blaVIM-2 (7.1%)] and K. pneumoniae [blaCTX-M (91.7%), blaTEM (83.3%), blaKPC (16.7%), blaNDM (12.5%), and blaOXA (4.2%)]. The results of testing the activity of ε-PL against the clinical isolates showed relatively high minimum inhibitory concentrations (MICs) for the P. aeruginosa (range: 8–64 µg/mL) and K. pneumoniae isolates (range: 16–32 µg/mL). These results suggest the need for prior optimization of ε-PL concerning its viability as an alternative to antibiotics for treating infections caused by P. aeruginosa and K. pneumoniae of clinical origin. It is noteworthy that, in the context of a low antibiotic discovery rate, ε-PL could play a significant role in this quest, considering its low toxicity and the unlikely development of resistance. Upon exposure to ε-PL, P. aeruginosa and K. pneumoniae isolates exhibited a reduction in biofilm production, with ε-PL concentration showing an inverse relationship, particularly in isolates initially characterized as strong or moderate producers, ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/38540251; hal-04520242; https://hal.inrae.fr/hal-04520242Test; https://hal.inrae.fr/hal-04520242/documentTest; https://hal.inrae.fr/hal-04520242/file/2023_De%20Sousa_Biomedicines.pdfTest; PUBMED: 38540251; WOS: 001191925300001 |
| DOI: | 10.3390/biomedicines12030638 |
| الإتاحة: | https://doi.org/10.3390/biomedicines12030638Test https://hal.inrae.fr/hal-04520242Test https://hal.inrae.fr/hal-04520242/documentTest https://hal.inrae.fr/hal-04520242/file/2023_De%20Sousa_Biomedicines.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.9A503E43 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/biomedicines12030638 |
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