دورية أكاديمية
A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera)
| العنوان: | A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera) |
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| المؤلفون: | Kerstin Seitz, Katharina Buczolich, Alžbeta Dikunová, Pavel Plevka, Karen Power, Till Rümenapf, Benjamin Lamp |
| المساهمون: | Seitz, Kerstin, Buczolich, Katharina, Dikunová, Alžbeta, Plevka, Pavel, Power, Karen, Rümenapf, Till, Lamp, Benjamin |
| سنة النشر: | 2019 |
| المجموعة: | IRIS Università degli Studi di Napoli Federico II |
| الوصف: | Among the many diseases compromising the well-being of the honey bee (Apis mellifera) the chronic paralysis syndrome of adult honey bees is one of the best described. The causative agent, chronic bee paralysis virus (CBPV), is a positive sense, single-stranded RNA virus with a segmented genome. Segment 1 encodes three putative open reading frames (ORFs), including the RNA-dependent RNA polymerase and other non-structural protein coding regions. Segment 2 encodes four putative ORFs, which contain the genes of supposed structural proteins. In this study, we established a reverse genetic system for CBPV by molecular cloning of DNA copies of both genome segments. CBPV rescue was studied in imago and honey bee pupae infection models. Virus replication and progeny virus production was only initiated when capped RNAs of both genome segments were injected in honey bees. As injection of these clonal RNAs caused clinical symptoms similar to wild-type CBPV infection, we conclude that the novel molecular clone fulfilled Koch's postulates. Our virus clone will enable in-depth analysis of CBPV pathogenesis and help to increase knowledge about this important honey bee disease. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000494945700041; volume:9; issue:1; firstpage:16274; journal:SCIENTIFIC REPORTS; https://hdl.handle.net/11588/922644Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85074683171 |
| DOI: | 10.1038/s41598-019-52822-1 |
| الإتاحة: | https://doi.org/10.1038/s41598-019-52822-1Test https://hdl.handle.net/11588/922644Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.98C2D91C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-019-52822-1 |
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