دورية أكاديمية
Viability of Escherichia coli K-12 DNA adenine methylase (dam) mutants requires increased expression of specific genes in the SOS regulon
| العنوان: | Viability of Escherichia coli K-12 DNA adenine methylase (dam) mutants requires increased expression of specific genes in the SOS regulon |
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| المؤلفون: | Peterson, Kenneth R., Wertman, Kenneth F., Mount, David W., Marinus, Martin G. |
| المساهمون: | Department of Biochemistry and Molecular Pharmacology |
| المصدر: | Molecular and general genetics : MGG ; 201 ; 1 ; 14-9 |
| سنة النشر: | 2022 |
| المجموعة: | University of Massachusetts, Medical School: eScholarship@UMMS |
| مصطلحات موضوعية: | DNA Repair, DNA, Bacterial, Escherichia coli, Genes, Genotype, Methyltransferases, Mutation, Rec A Recombinases, Site-Specific DNA-Methyltransferase (Adenine-Specific), Species Specificity, Transduction, Genetic, beta-Galactosidase, Biochemistry, Biophysics, and Structural Biology |
| الوصف: | We have examined the level of expression of the SOS regulon in cells lacking DNA adenine methylase activity (dam-). Mud (Ap, lac) fusions to several SOS operons (recA, lexA, uvrA, uvrB, uvrD, sulA, dinD and dinF) were found to express higher levels of beta-galactosidase in dam- strains than in isogenic dam+ strains. The attempted construction of dam- strains that were also mutant in one of several SOS genes indicated that the viability of methylase-deficient strains correlates with the inactivation of the SOS repressor (LexA protein). Consistent with this, the wild-type functions of two LexA-repressed genes (recA and ruv) appear to be required for dam- strain viability. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Link to Article in PubMed; http://dx.doi.org/10.1007/BF00397979Test; Mol Gen Genet. 1985;201(1):14-9.; 0026-8925 (Print); 3932821; http://hdl.handle.net/20.500.14038/26083Test; https://escholarship.umassmed.edu/bmp_pp/24Test; 690850; bmp_pp/24 |
| الإتاحة: | https://doi.org/20.500.14038/26083Test https://doi.org/10.1007/BF00397979Test https://hdl.handle.net/20.500.14038/26083Test https://escholarship.umassmed.edu/bmp_pp/24Test |
| رقم الانضمام: | edsbas.98BE1171 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |