دورية أكاديمية
Simultaneous Purification of Human Interferon Alpha-2b and Serum Albumin Using Bioprivileged Fluorinated Ionic Liquid-Based Aqueous Biphasic Systems
| العنوان: | Simultaneous Purification of Human Interferon Alpha-2b and Serum Albumin Using Bioprivileged Fluorinated Ionic Liquid-Based Aqueous Biphasic Systems |
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| المؤلفون: | Carvalho, Sara F., Pereiro, Ana B., Araújo, João M. M. |
| المساهمون: | LAQV@REQUIMTE, DQ - Departamento de Química |
| سنة النشر: | 2024 |
| المجموعة: | Repositório da Universidade Nova de Lisboa (UNL) |
| مصطلحات موضوعية: | aqueous biphasic systems, bioactive proteins, bioprivileged fluorinated ionic liquids, BSA, human IFN-α2b, purification, serum albumin, Catalysis, Molecular Biology, Spectroscopy, Computer Science Applications, Physical and Theoretical Chemistry, Organic Chemistry, Inorganic Chemistry, SDG 3 - Good Health and Well-being |
| الوصف: | Publisher Copyright: © 2024 by the authors ; Interferon alpha-2b (IFN- (Formula presented.) 2b) is an essential cytokine widely used in the treatment of chronic hepatitis C and hairy cell leukemia, and serum albumin is the most abundant plasma protein with numerous physiological functions. Effective single-step aqueous biphasic system (ABS) extraction for the simultaneous purification of IFN- (Formula presented.) 2b and BSA (serum albumin protein) was developed in this work. Effects of the ionic liquid (IL)-based ABS functionalization, fluorinated ILs (FILs; [C (Formula presented.) C (Formula presented.) Im][C (Formula presented.) F (Formula presented.) SO (Formula presented.)] and [N (Formula presented.)][C (Formula presented.) F (Formula presented.) SO (Formula presented.)]) vs. mere fluoro-containing IL ([C (Formula presented.) C (Formula presented.) Im][CF (Formula presented.) SO (Formula presented.)]), in combination with sucrose or [N (Formula presented.)][H (Formula presented.) PO (Formula presented.)] (well-known globular protein stabilizers), or high-charge-density salt K (Formula presented.) PO (Formula presented.) were investigated. The effects of phase pH, phase water content (%wt), phase composition (%wt), and phase volume ratio were investigated. The phase pH was found to have a significant effect on IFN- (Formula presented.) 2b and BSA partition. Experimental results show that simultaneous single-step purification was achieved with a high yield (extraction efficiency up to 100%) for both proteins and a purification factor of IFN- (Formula presented.) 2b high in the enriched IFN- (Formula presented.) 2b phase (up to 23.22) and low in the BSA-enriched phase (down to 0.00). SDS-PAGE analysis confirmed the purity of both recovered proteins. The stability and structure of IFN- (Formula presented.) 2b and BSA were preserved or even improved (FIL-rich phase) during the purification step, as evaluated by CD spectroscopy and DSC. Binding studies of IFN- (Formula presented.) 2b and BSA with the ABS ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1661-6596 |
| العلاقة: | info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F139677%2F2018/PT; info:eu-repo/grantAgreement/FCT/CEEC IND 3ed/2020.00835.CEECIND%2FCP1586%2FCT0014/PT; info:eu-repo/grantAgreement/FCT/CEEC IND4ed/2021.01432.CEECIND%2FCP1657%2FCT0044/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0008%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT; PURE: 86390388; PURE UUID: 3625c775-47d4-4d39-9e5b-94a14b47ab30; Scopus: 85187723358; WOS: 001183284500001; PubMed: 38473998; PubMedCentral: PMC10931833; ORCID: /0000-0002-8648-7539/work/157689447; http://hdl.handle.net/10362/166248Test; https://doi.org/10.3390/ijms25052751Test |
| DOI: | 10.3390/ijms25052751 |
| الإتاحة: | https://doi.org/10.3390/ijms25052751Test http://hdl.handle.net/10362/166248Test |
| حقوق: | openAccess |
| رقم الانضمام: | edsbas.97CA6114 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 16616596 |
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| DOI: | 10.3390/ijms25052751 |