التفاصيل البيبلوغرافية
| العنوان: |
Establishment of Realtime RT-PCR Assay to Detect Polio Virus in the Acute Flaccid Paralysis Laboratory Surveillance |
| المؤلفون: |
Susanti, N. (Nike), Setiawaty, V. (Vivi) |
| المصدر: |
Health Science Journal of Indonesia |
| بيانات النشر: |
National Institute of Health Research and Development, Indonesian Ministry of Health |
| سنة النشر: |
2016 |
| المجموعة: |
neliti (Indonesia's Think Tank Database) |
| مصطلحات موضوعية: |
Indonesia, rRT-PCR, Identification, Poliovirus, ITD |
| الوصف: |
Background: The last indigenous polio was detected in 1995 but the threat of wild type polio viruses and themutation of Oral Polio Vaccine into Vaccine-Derived Poliovirus still continue. Since 1991, WHO has developedAcute Flaccid Paralysis (AFP) laboratory based surveillance. In 2014, the polioviruses identification by real-timeReverse Transcriptase Polymerase Chain Reaction (rRT-PCR), has been introduced to National Polio Laboratory(NPL) Center for Biomedical and Basic Technology of Health. The objective of the rRT-PCR application is to havefaster and better diagnostic methods to monitor the circulation and mutation of polio viruses.Methods: Isolate tested by rRT-PCR using a combination of primers and probe mentioned by WHO manual.The viral RNA is converted to cDNA using reverse transcriptase and amplified in a PCR reaction using Taqpolymerase. The PCR products are detected and identified by hybridization with specific probes. The combinationof primers and probes will result in the serotype identification and intratypic differentiation of poliovirus isolates.Results: In 2014 NPL Jakarta received 604 AFP cases through the surveillance system, five cases foundpositive for polio viruses by culture. All of the specimens were positive for polio vaccine viruses. Twocases were polio virus type P2 (40%), one cases polio virus type P1 (20%), 1 case polio virus type P3(20%) and one case mix polio viruses type P1+P2 (20%).Conclusion: The real-time PCR assay was able to help the identification of polio viruses rapidly in Jakartalab. The test can be utilized for monitoring the population routinely immunized with OPV. (Health ScienceJournal of Indonesia 2016;7:27-31) |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
https://www.neliti.com/publications/62636/establishment-of-realtime-rt-pcr-assay-to-detect-polio-virus-in-the-acute-flacciTest |
| الإتاحة: |
https://www.neliti.com/publications/62636/establishment-of-realtime-rt-pcr-assay-to-detect-polio-virus-in-the-acute-flacciTest |
| حقوق: |
(c) Health Science Journal of Indonesia, 2016 |
| رقم الانضمام: |
edsbas.974A8B7A |
| قاعدة البيانات: |
BASE |
