دورية أكاديمية
Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics.
| العنوان: | Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics. |
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| المؤلفون: | Rojas-Gómez, Amelia, Dosil, Sara G, Chichón, Francisco J, Fernández-Gallego, Nieves, Ferrarini, Alessia, Calvo, Enrique, Calzada-Fraile, Diego, Requena, Silvia, Otón, Joaquin, Serrano, Alvaro, Tarifa, Rocio, Arroyo, Montserrat, Sorrentino, Andrea, Pereiro, Eva, Vazquez, Jesus, Valpuesta, José M, Sánchez-Madrid, Francisco, Martín-Cófreces, Noa B |
| بيانات النشر: | Wiley |
| سنة النشر: | 2023 |
| المجموعة: | REPISALUD (REPositorio Institucional en SALUD del Instituto de Salud Carlos III - ISCIII) |
| مصطلحات موضوعية: | Kinesins, Extracellular Vesicles, Chaperonin Containing TCP-1, Lipid Metabolism, Lipids |
| الوصف: | Cell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modifications, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganelle contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT. ; Comunidad de Madrid, Grant/Award Numbers:S2022/BMD-7209-INTEGRAMUNE-CM,P2022/BMD-7333; Carlos III Institute ofHealth-Fondo de Investigacion Sanitaria,Grant/Award Numbers: PRB3 (IPT17/0019 -ISCIII-SGEFI / ERDF), CIBER Cardiovascular(CIBERCV); La Caixa Banking Foundation,Grant/Award Numbers: HR17-00016, HR17-00247,HR22-00253, HR22-00722; consorcio para laconstrucción equipamiento y explotación dellaboratorio de luz sincrotrón, Grant/AwardNumbers: 2015021148, 2016021638; Agencia Estatalde Investigación, Grant/Award Numbers:PDC2021-121719-I00, PLEC2022-009298,PID-2020-120412RB-I00, PGC2018-097019-B-I00,PID2021-122348NB-I00, PID2019-105872GB-I00/AEI/10.13039/501100011033. ; Sí |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2001-3078 |
| العلاقة: | J Extracell Vesicles. 2023 Jun;12(6):e12333.; http://hdl.handle.net/20.500.12105/16733Test; Journal of extracellular vesicles |
| DOI: | 10.1002/jev2.12333 |
| الإتاحة: | https://doi.org/20.500.12105/16733Test https://doi.org/10.1002/jev2.12333Test https://hdl.handle.net/20.500.12105/16733Test |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ ; Atribución 4.0 Internacional ; open access |
| رقم الانضمام: | edsbas.972784C9 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20013078 |
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| DOI: | 10.1002/jev2.12333 |