دورية أكاديمية
EVALUATION OF AST FAST ES/NF AGAR MEDIUM FOR RAPID DETECTION OF IMIPENEM RESISTANCE IN CLINICAL KLEBSIELLA PNEUMONIAE AND ACINETOBACTER BAUMANNII ISOLATES ; KLİNİK KLEBSIELLA PNEUMONIAE VE ACINETOBACTER BAUMANNII İZOLATLARINDA İMİPENEM DİRENCİNİN HIZLI TESPİTİNDE AST FAST ES/NF AGAR BESİYERİNİN DEĞERLENDİRİLMESİ
| العنوان: | EVALUATION OF AST FAST ES/NF AGAR MEDIUM FOR RAPID DETECTION OF IMIPENEM RESISTANCE IN CLINICAL KLEBSIELLA PNEUMONIAE AND ACINETOBACTER BAUMANNII ISOLATES ; KLİNİK KLEBSIELLA PNEUMONIAE VE ACINETOBACTER BAUMANNII İZOLATLARINDA İMİPENEM DİRENCİNİN HIZLI TESPİTİNDE AST FAST ES/NF AGAR BESİYERİNİN DEĞERLENDİRİLMESİ |
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| المؤلفون: | Temel, A., Tekintaş, Y., Hoşgör, Limoncu, M., Çilli, F. |
| بيانات النشر: | University of Ankara |
| سنة النشر: | 2023 |
| المجموعة: | Ege University Institutional Repository |
| مصطلحات موضوعية: | Acinetobacter baumannii, AST fast agar, chromogenic medium, disk diffusion method, Klebsiella pneumoniae, imipenem, antibiotic resistance, antibiotic sensitivity, Article, bacterium detection, bacterium isolation, broth dilution, controlled study, disk diffusion, Gram negative bacterium, human, nonhuman, polymerase chain reaction, sensitivity and specificity, zone of inhibition |
| الوصف: | Objective: Resistant gram-negative microorganisms, especially Klebsiella pneumoniae and Acinetobacter baumannii, cause life-threatening infections. Hence, it is critical to determine the antibiotic susceptibility of clinical isolates rapidly and accurately. Nowadays, carbapenem resistance is determined by phenotypic/genotypic methods. For phenotypic methods (disk diffusion, microdilution), differences in specificity and sensitivity can often occur. Since molecular methods such as polymerase chain reaction giving relatively more reliable results, are labor-intensive and costly, new fast, reliable and economical methods are needed. Material and Method: The performance of chromogenic AST Fast ES/NF agar for the EUCAST standard disk diffusion method was investigated. Four K. pneumoniae and five A. baumannii isolates, which were determined to be carbapenem resistant by molecular method and automated system results, were included in the study. Result and Discussion: The inhibition zone diameters were became measurable within 4-6 hours in AST Fast ES/NF and the susceptibility categories were interpreted according to the manufacturer's recommendations. The results of disk diffusion test performed with MHA, were largely consistent with the results obtained with the AST Fast ES/NF. Considering that the incubation period recommended by EUCAST in the standard disk diffusion test method is 16-20 hours and the sensitivity category is measured at the end of this period, it is thought that the chromogenic medium may provide a significant advantage. © 2023 Authors. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | Turkish |
| تدمد: | 1015-3918 |
| العلاقة: | Ankara Universitesi Eczacilik Fakultesi Dergisi; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.33483/jfpau.1230197Test; https://hdl.handle.net/11454/83370Test; 47; 2-s2.0-85150916645 |
| DOI: | 10.33483/jfpau.1230197 |
| الإتاحة: | https://doi.org/10.33483/jfpau.1230197Test https://hdl.handle.net/11454/83370Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.96F027B3 |
| قاعدة البيانات: | BASE |
| تدمد: | 10153918 |
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| DOI: | 10.33483/jfpau.1230197 |