دورية أكاديمية
Novel Siphoviridae phage PMBT4 belonging to the group b Lactobacillus delbrueckii subsp. bulgaricus phages
| العنوان: | Novel Siphoviridae phage PMBT4 belonging to the group b Lactobacillus delbrueckii subsp. bulgaricus phages |
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| المؤلفون: | Sprotte, Sabrina, Fagbemigun, Olakunle, Brinks, Erik, Cho, Gyu-Sung, Casey, Eoghan, Oguntoyinbo, Folarin A., Neve, Horst, Mahony, Jennifer, van Sinderen, Douwe, Franz, Charles M.A.P. |
| بيانات النشر: | Elsevier B.V. |
| سنة النشر: | 2021 |
| المجموعة: | University College Cork, Ireland: Cork Open Research Archive (CORA) |
| مصطلحات موضوعية: | Lactobacillus delbrueckii phage, Genome, Group b, KIS element |
| الوصف: | A novel Lactobacillus delbrueckii bacteriophage PMBT4 was isolated from the Nigerian fermented milk product nono. The phage possesses a long and thin, non-contractile tail and an isometric head, indicating that it belongs to the Siphoviridae family. A neck passage structure (`collar`), previously hypothesized to be encoded by two genes located in the Lactobacillus delbrueckii phage LL-K insertion sequence (KIS) element, as well as in two additional Lb. delbrueckii phages Ld17 and Ld25A, could also be observed on an estimated 1–5% of phage particles by transmission electron microscopy. However, neither mapping of high throughput sequencing data to KIS element genes from Lb. delbrueckii phages LL-K, Ld17 and Ld25A nor PCR amplification of the KIS element genes could corroborate the presence of these genes in the PMBT4 genome. The PMBT4 genome consists of 31,399 bp with a mol% GC content of 41.6 and exhibits high (95–96%) sequence homologies to Lb. delbrueckii phages c5, Ld3, Ld25A and Ld17, which assigned PMBT4 as a new member of this genus, i.e. the Cequinquevirus genus. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; application/vnd.openxmlformats-officedocument.wordprocessingml.document |
| اللغة: | English |
| تدمد: | 1872-7492 0168-1702 |
| العلاقة: | 198635; Sprotte, S., Fagbemigun, O.,Brinks, E., Cho, G.-S., Casey, E., Oguntoyinbo, F. A., Neve, H., Mahony, J., van Sinderen, D. and Franz, C. M.A.P. (2021) 'Novel Siphoviridae phage PMBT4 belonging to the group b Lactobacillus delbrueckii subsp. bulgaricus phages', Virus Research, 308, 198635 (7pp). doi:10.1016/j.virusres.2021.198635; Virus Research; https://hdl.handle.net/10468/12399Test; 308 |
| DOI: | 10.1016/j.virusres.2021.198635 |
| الإتاحة: | https://doi.org/10.1016/j.virusres.2021.198635Test https://hdl.handle.net/10468/12399Test |
| حقوق: | © 2021, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license. |
| رقم الانضمام: | edsbas.968EB301 |
| قاعدة البيانات: | BASE |
| تدمد: | 18727492 01681702 |
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| DOI: | 10.1016/j.virusres.2021.198635 |