دورية أكاديمية
Evaluation of virulence factors in enterococcus species
| العنوان: | Evaluation of virulence factors in enterococcus species |
|---|---|
| المؤلفون: | Mete, Ergun, Kaleli, I, Cevahir, Nural, Demir, Melek, Akkaya, Yüksel, KiriÅŸ Satılmış, Özgün |
| بيانات النشر: | ANKARA MICROBIOLOGY SOC |
| سنة النشر: | 2017 |
| المجموعة: | Pamukkale University Repository / Pamukkale Üniversitesi Açık Erişim Arşivi |
| مصطلحات موضوعية: | Enterococcus spp., virulence factors, antimicrobial resistance, vancomycin resistant enterococci |
| الوصف: | Enterococci have recently become important due to their increased isolation rates in community-based and nosocomial infections and resistance to many antibiotics, including glycopeptides. The aim of this study was to evaluate the antimicrobial susceptible patterns and virulence factors of various clinical specimens; urine (n= 149), blood (n= 38), wound (n= 17), stool (n= 13), and other (n= 12) with a total of 229 enterococci including 138 E. faecalis and 91 E. faecium isolates. Aggregation factor (AF), enterococcus surface protein (esp), cytolysins and gelatinase encoding genes (asa1, esp, cylM, cylBcyl A, cylll, cylls, gelE, respectively) were investigated by molecular methods. Haemolysin production and gelatinase were studied phenotypically. A total of 30 isolates, 29 of E. faecium and one of E. faecalis isolates were resistant to vancomycin. High-level gentamicin and high-level streptomycin resistance in E. faecalis were 40.7% and 63.7% however, they were 47.1% and 55.8% in E. faecalis isolates. All strains were susceptible to linezolid. Ampicillin, penicillin and vancomycin resistance in E. faecium isolates were found to be higher than E. faecalis isolates (p= 0.001, p= 0.008 and p 0.001). Asa1 (p 0.001), cylll (p= 0.002) and cylls (p 0.001) as well as gelatinase activity in isolates of E. faecalis were significantly higher than the isolates of E. faecium (p 0.001). The most common virulence genes in our study were asa1 gene (45%), cyLs gene (33.2%) and esp gene (32.3%). Ciprofloxacin resistance in cylLL and cyLs gene positive isolates of E. faecalis were significantly higher compared to isolates that do not contain these genes (p= 0.035 and p= 0.047). Likewise, haemolysin producing E. faecium isolates were significantly more resistant to vancomycin compared to isolates that do not produce hemolysin (p 0.001). When the virulence factors of vancomycin resistant and susceptible isolates were compared, the esp gene level in VRE E. faecium isolates was found to be 24.1%, while no esp gene was found in VRE E. ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | Turkish |
| تدمد: | 0374-9096 |
| العلاقة: | MIKROBIYOLOJI BULTENI; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://hdl.handle.net/11499/23865Test; https://doi.org/10.5578/mb.53992Test; 51; 101; 114; 2-s2.0-85021661739; WOS:000402308400001 |
| DOI: | 10.5578/mb.53992 |
| الإتاحة: | https://doi.org/10.5578/mb.53992Test https://hdl.handle.net/11499/23865Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.965CBE1F |
| قاعدة البيانات: | BASE |
| تدمد: | 03749096 |
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| DOI: | 10.5578/mb.53992 |