التفاصيل البيبلوغرافية
| العنوان: |
Osteoblastic differentiation and stress response of human mesenchymal stem cells exposed to alternating current electric fields |
| المؤلفون: |
Hronik-Tupaj, Marie, Rice, William L, Cronin-Golomb, Mark, Kaplan, David L, Georgakoudi, Irene |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2011 |
| المجموعة: |
BioMed Central |
| الوصف: |
Background Electric fields are integral to many biological events, from maintaining cellular homeostasis to embryonic development to healing. The application of electric fields offers substantial therapeutic potential, while optimal dosing regimens and the underlying mechanisms responsible for the positive clinical impact are poorly understood. Methods The purpose of this study was to track the differentiation profile and stress response of human bone marrow derived mesenchymal stem cells (hMSCs) undergoing osteogenic differentiation during exposure to a 20 mV/cm, 60 kHz electric field. Morphological and biochemical changes were imaged using endogenous two-photon excited fluorescence (TPEF) and quantitatively assessed through eccentricity calculations and extraction of the redox ratio from NADH, FAD and lipofuscin contributions. Real time reverse transcriptase-polymerase chain reactions (RT-PCR) were used to track osteogenic differentiation markers, namely alkaline phosphatase (ALP) and collagen type 1 (col1), and stress response markers, such as heat shock protein 27 (hsp27) and heat shock protein 70 (hsp70). Comparisons of collagen deposition between the stimulated hMSCs and controls were examined through second harmonic generation (SHG) imaging. Results Quantitative differences in cell morphology, as described through an eccentricity ratio, were found on days 2 and days 5 (p < 0.05) in samples exposed to the electric field. A delayed but two fold increase in ALP and col1 transcript was detected by week 2 (p < 0.05) in differentiating hMSCs exposed to an electric field in comparison to the nonstimulated controls. Upregulation in stress marker, hsp27, and type 1 collagen deposition were correlated with this response. Increases in NADH, FAD, and lipofuscin were traced in the stimulation group during the first week of field exposure with differences statistically significant on day 10 (p < 0.05). Changes in hsp27 expression correlate well with changes in lipofuscin detected in the stimulation ... |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| العلاقة: |
http://www.biomedical-engineering-online.com/content/10/1/9Test |
| الإتاحة: |
http://www.biomedical-engineering-online.com/content/10/1/9Test |
| حقوق: |
Copyright 2011 Hronik-Tupaj et al; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.96390FCE |
| قاعدة البيانات: |
BASE |
