التفاصيل البيبلوغرافية
| العنوان: |
TRIM7/RNF90 promotes autophagy via regulation of ATG7 ubiquitination during L. monocytogenes infection |
| المؤلفون: |
Jie Wang (16762), Xiao Qin (383345), Yulu Huang (8969363), Qunmei Zhang (14306779), Jinyong Pei (14306782), Yi Wang (32470), Idan Goren (7352393), Shujun Ma (8525823), Zhishan Song (14306785), Yanzi Liu (8525826), Hongxia Xing (14306788), Hui Wang (30400), Bo Yang (104813) |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
Biochemistry, Medicine, Microbiology, Cell Biology, Immunology, Science Policy, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, autophagosome accumulation, autophagy-related genes, intracellular bacteria, posttranslational modification, ring finger proteins, E3 ligase |
| الوصف: |
L. monocytogenes is a widely used infection model for the research on pathogenesis and host defense against gram-positive intracellular bacteria. Emerging evidence indicates that posttranslational modifications play a critical role in the regulation of macroautophagy/autophagy. However, little is known about the posttranslational modifications of ATG7, the essential protein in the autophagy process. In this study, we demonstrated that the RING-type E3 ligase TRIM7/RNF90 positively regulated autophagosome accumulation by promoting the ubiquitination of ATG7 at K413, thereby affecting L. monocytogenes infection. TRIM7 expression was induced by a variety range of conditions, including starvation, rapamycin stimulation, and L. monocytogenes infection. TRIM7 deficiency in mice or cells resulted in elevated innate immune responses and increased L. monocytogenes infection. ATG7 was associated with TRIM7 and the positive regulatory role of TRIM7 in L. monocytogenes infection-, starvation- or rapamycin-induced autophagosome accumulation was suggested by TRIM7 deficiency, TRIM7 overexpression, and TRIM7 knockdown. Further mechanistic investigation indicated that TRIM7 promoted the K63-linked ubiquitination of ATG7 at K413 and ubiquitination at this site was required for the function of ATG7 in autophagy and L. monocytogenes infection. Thus, our findings suggested a new regulator in intracellular bacterial infection and autophagy, with a novel posttranslational modification targeting ATG7. This research may expand our understanding of host anti-bacterial defense and the role of autophagy in intracellular bacterial infection. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/TRIM7_RNF90_promotes_autophagy_via_regulation_of_ATG7_ubiquitination_during_i_L_monocytogenes_i_infection/21786607Test |
| DOI: |
10.6084/m9.figshare.21786607.v1 |
| الإتاحة: |
https://doi.org/10.6084/m9.figshare.21786607.v1Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.9584E405 |
| قاعدة البيانات: |
BASE |
