دورية أكاديمية
Cytotoxicity Assessment of Ma-huang (Ephedra) under Different Conditions of Preparation
| العنوان: | Cytotoxicity Assessment of Ma-huang (Ephedra) under Different Conditions of Preparation |
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| المؤلفون: | Lee, M. K., Cheng, B. W. H., Che, C. T., Hsieh, D. P. H. |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2000 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Safety Evaluation |
| الوصف: | Ma-huang is a traditional Chinese medicinal herb derived from Ephedra sinica Stapf and other Ephedra species, used to treat asthma, nose and lung congestion, and fever with anhidrosis. It contains 0.5–2.5% by weight of total alkaloids, of which ephedrine accounts for 30 to 90%. Recently, large amounts of ma-huang were used as a source of ephedrine in many dietary supplements formulated for weight reduction, because ephedrine has been found effective in inducing weight loss in diet-restricted obese patients. However, indiscriminate consumption of ma-huang–containing products has resulted in many cases of poisoning, some of which were fatal. The objective of this study is to investigate the relative toxicity of ma-huang extracted under different conditions. The toxicities of various extracts were assayed using MTT colorimetry on a battery of cell lines, while ephedrine alkaloids were analyzed with HPLC. The results are summarized as follows. (1) The cytotoxicity of all ma-huang extracts could not be totally accounted for by their ephedrine contents, suggesting the presence of other toxins in the extracts. (2) Grinding was a significant condition enhancing the toxicity of the extracts. (3) The relatively high sensitivity of the Neuro-2a cell line to the toxicity of ma-huang extracts suggests that the toxic principles were acting on neuronal cells. (4) One condition to produce a ma-huang extract with high ephedrine-to-toxins ratio would be to boil the whole herb for two h. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://toxsci.oxfordjournals.org/cgi/content/short/56/2/424Test; http://dx.doi.org/10.1093/toxsci/56.2.424Test |
| DOI: | 10.1093/toxsci/56.2.424 |
| الإتاحة: | https://doi.org/10.1093/toxsci/56.2.424Test http://toxsci.oxfordjournals.org/cgi/content/short/56/2/424Test |
| حقوق: | Copyright (C) 2000, Society of Toxicology |
| رقم الانضمام: | edsbas.954A889C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/toxsci/56.2.424 |
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