دورية أكاديمية
A Comparison of Gene Expression Profiles between Glucocorticoid Responder and Non-Responder Bovine Trabecular Meshwork Cells Using RNA Sequencing
| العنوان: | A Comparison of Gene Expression Profiles between Glucocorticoid Responder and Non-Responder Bovine Trabecular Meshwork Cells Using RNA Sequencing |
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| المؤلفون: | Bermudez, Jaclyn Y., Webber, Hannah C., Brown, Bartley, Braun, Terry A., Clark, Abbot F., Mao, Weiming |
| المصدر: | PLoS One |
| بيانات النشر: | PLOS |
| سنة النشر: | 2022 |
| المجموعة: | UNTHSC Scholarly Repository (University. of North Texas Health Science Center) |
| مصطلحات موضوعية: | Animals, Cattle, Computational Biology / methods, Gene Expression Profiling, Gene Expression Regulation / drug effects, Glucocorticoids / pharmacology, High-Throughput Nucleotide Sequencing, Protein Interaction Mapping, Sequence Analysis, RNA, Signal Transduction, Trabecular Meshwork / cytology, Trabecular Meshwork / metabolism, Transcriptome |
| الوصف: | The most common ocular side effect of glucocorticoid (GC) therapy is GC-induced ocular hypertension (OHT) and GC-induced glaucoma (GIG). GC-induced OHT occurs in about 40% of the general population, while the other 60% are resistant. This study aims to determine the genes and pathways involved in differential GC responsiveness in the trabecular meshwork (TM). Using paired bovine eyes, one eye was perfusion-cultured with 100nM dexamethasone (DEX), while the fellow eye was used to establish a bovine TM (BTM) cell strain. Based on maximum IOP change in the perfused eye, the BTM cell strain was identified as a DEX-responder or non-responder strain. Three responder and three non-responder BTM cell strains were cultured, treated with 0.1% ethanol or 100nM DEX for 7 days. RNA and proteins were extracted for RNA sequencing (RNAseq), qPCR, and Western immunoblotting (WB), respectively. Data were analyzed using the human and bovine genome databases as well as Tophat2 software. Genes were grouped and compared using Student's t-test. We found that DEX induced fibronectin expression in responder BTM cells but not in non-responder cells using WB. RNAseq showed between 93 and 606 differentially expressed genes in different expression groups between responder and non-responder BTM cells. The data generated by RNAseq were validated using qPCR. Pathway analyses showed 35 pathways associated with differentially expressed genes. These genes and pathways may play important roles in GC-induced OHT and will help us to better understand differential ocular responsiveness to GCs. ; This work was funded by the National Eye Institute R21EY023048 (W.M.), https://nei.nih.govTest/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This work was also funded by a National Institutes of Health training grant in the Neurobiology of Aging T32 AG20494 (J.Y.B & H.C.W.), https://www.nih.govTest/. The funders had no role in study design, data collection and analysis, decision to ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| تدمد: | 1932-6203 |
| العلاقة: | https://doi.org/10.1371/journal.pone.0169671Test; Bermudez, J. Y., Webber, H. C., Brown, B., Braun, T. A., Clark, A. F., & Mao, W. (2017). A Comparison of Gene Expression Profiles between Glucocorticoid Responder and Non-Responder Bovine Trabecular Meshwork Cells Using RNA Sequencing. PloS one, 12(1), e0169671. https://doi.org/10.1371/journal.pone.0169671Test; https://hdl.handle.net/20.500.12503/31723Test; 12 |
| الإتاحة: | https://doi.org/20.500.12503/31723Test https://doi.org/10.1371/journal.pone.0169671Test https://hdl.handle.net/20.500.12503/31723Test |
| حقوق: | Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0Test/ ; © 2017 Bermudez et al. |
| رقم الانضمام: | edsbas.9521808B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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