التفاصيل البيبلوغرافية
| العنوان: |
Ex Vivo Mitochondrial Respiration Parallels Biochemical Response to Ibrutinib in CLL Cells |
| المؤلفون: |
Subir Roy Chowdhury, Cheryl Peltier, Sen Hou, Amandeep Singh, James B. Johnston, Spencer B. Gibson, Aaron J. Marshall, Versha Banerji |
| المصدر: |
Cancers; Volume 13; Issue 2; Pages: 354 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2021 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
mitochondrial respiration, chronic lymphocytic leukemia (CLL), B-cell receptor (BCR), ibrutinib (IB), Bruton tyrosine kinase (BTK) inhibitor, plasma chemokine (C-C motif) ligands 3 and 4 (CCL3 and CCL4), β-2 microglobulin (β-2 M), lactate dehydrogenase (LDH) |
| الوصف: |
Mitochondrial respiration is becoming more commonly used as a preclinical tool and potential biomarker for chronic lymphocytic leukemia (CLL) and activated B-cell receptor (BCR) signaling. However, respiration parameters have not been evaluated with respect to dose of ibrutinib given in clinical practice or the effect of progression on ibrutinib treatment on respiration of CLL cells. We evaluated the impact of low and standard dose ibrutinib on CLL cells from patients treated in vivo on mitochondrial respiration using Oroboros oxygraph. Cytokines CCL3 and CCL4 were evaluated using the Mesoscale. Western blot analysis was used to evaluate the BCR and apoptotic pathways. We observed no difference in the mitochondrial respiration rates or levels of plasma chemokine (C-C motif) ligands 3 and 4 (CCL3/CCL4), β-2 microglobulin (β-2 M) and lactate dehydrogenase (LDH) between low and standard doses of ibrutinib. This may confirm why clinical observations of the safety and efficacy of low dose ibrutinib are observed in practice. Of interest, we also observed that the mitochondrial respiration of CLL cells paralleled the increase in β-2 M and LDH at progression. Our study further supports mitochondrial respiration as a biomarker for response and progression on ibrutinib in CLL cells and a valuable pre-clinical tool. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Cancer Therapy; https://dx.doi.org/10.3390/cancers13020354Test |
| DOI: |
10.3390/cancers13020354 |
| الإتاحة: |
https://doi.org/10.3390/cancers13020354Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.9511F4FE |
| قاعدة البيانات: |
BASE |
