دورية أكاديمية
Is it possible to use expired tubes for routine biochemical analysis in dogs?
| العنوان: | Is it possible to use expired tubes for routine biochemical analysis in dogs? |
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| المؤلفون: | Domingos, Margarida Canelas, Medaille, Christine, Concordet, Didier, Briend-Marchal, Alexandra |
| المساهمون: | Vebiotel - Laboratoire de Biologie Vétérinaire, ToxAlim (ToxAlim), Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT) |
| المصدر: | ISSN: 0275-6382 ; Veterinary Clinical Pathology ; https://hal.science/hal-01191328Test ; Veterinary Clinical Pathology, 2012, 41 (2), pp.266-271. ⟨10.1111/j.1939-165X.2012.00424.x⟩. |
| بيانات النشر: | HAL CCSD Wiley-Blackwell |
| سنة النشر: | 2012 |
| المجموعة: | Université Toulouse III - Paul Sabatier: HAL-UPS |
| مصطلحات موضوعية: | ERRORS, Canine, clinical chemistry, expiration date, heparin, plasma, LABORATORY MEDICINE, CANINE PLASMA, BLOOD, TEMPERATURE, PARAMETERS, [SDV]Life Sciences [q-bio] |
| الوصف: | Background Expired collection tubes may be used inadvertently and resampling is not always possible. To date, studies have not been conducted in veterinary medicine to determine whether or not biochemical measurements obtained from specimens collected into expired tubes are accurate enough for clinical decision-making. Objectives The aims of this preliminary study were to assess the impact of measuring routine plasma biochemical analytes in canine specimens collected in expired tubes and to investigate the relationship between post-expiration time and the magnitude of errors. Methods Blood specimens were collected from 61 dogs and aliquoted equally into tubes containing lithium heparin and gel. One tube was within the expiration date, and the other tube was up to 11months post-expiration. Plasma was separated within 1hour of specimen collection, and concentrations of urea, creatinine, total protein, albumin, total bilirubin, cholesterol, triglycerides, magnesium, calcium, phosphates, sodium, potassium, chloride, total CO2 , and fructosamine and activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase (ALP), ?-glutamyltransferase, lactate dehydrogenase, creatine kinase, amylase, and lipase were analyzed immediately and results compared. Results For most analytes there was no significant difference between results from specimens collected in non-expired and expired tubes. For ALP and lipase activities and fructosamine and total CO2 concentrations, significant differences were found, and results obtained for fructosamine and total CO2 from specimens in expired tubes may have led to erroneous interpretations. The effect of time since expiration was constant over time. Conclusions When specimens are processed within 1hour of collection, results of routine biochemical measurements of blood collected in lithium heparin tubes remain clinically valid for up to 11months after expiration of tubes for the majority of analytes, except for ALP, lipase, fructosamine, and total CO2 . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/22551094; hal-01191328; https://hal.science/hal-01191328Test; https://hal.science/hal-01191328/documentTest; https://hal.science/hal-01191328/file/VET%20%20CLIN%20PATHOL%20Is%20it%20possible%20to%20use%20expired%20tubes%20for%20routine%20biochemical%20-2012_%7BBAADBEA4-264D-41AC-91F3-FC86E460FD3E%7D.pdfTest; PRODINRA: 178305; PUBMED: 22551094; WOS: 000304764300015 |
| DOI: | 10.1111/j.1939-165X.2012.00424.x |
| الإتاحة: | https://doi.org/10.1111/j.1939-165X.2012.00424.xTest https://hal.science/hal-01191328Test https://hal.science/hal-01191328/documentTest https://hal.science/hal-01191328/file/VET%20%20CLIN%20PATHOL%20Is%20it%20possible%20to%20use%20expired%20tubes%20for%20routine%20biochemical%20-2012_%7BBAADBEA4-264D-41AC-91F3-FC86E460FD3E%7D.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.94AE2B5D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1939-165X.2012.00424.x |
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