التفاصيل البيبلوغرافية
| العنوان: |
The Effects of Red Light on Mammalian Sperm Rely upon the Color of the Straw and the Medium Used |
| المؤلفون: |
Catalán, Jaime, Yánez-Ortiz, Iván, Gacem, Sabrina, Papas, Marion, Bonet, Sergi, Rodríguez Gil, Joan Enric, Yeste Oliveras, Marc, Miró, Jordi |
| سنة النشر: |
2021 |
| المجموعة: |
Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
| مصطلحات موضوعية: |
Cavalls, Espermatozoides, Horse, Sperm, Red light irradiation, Extender, Straw |
| الوصف: |
Altres ajuts: PFCHA/2017/72180128 ; Altres ajuts: PBIP/CZ02-000507-2019 ; Several studies have shown that the exposure of semen to red light improves sperm quality and fertilizing ability, which could improve the efficiency of assisted reproductive techniques with irradiated semen. However, despite being considered as possible sources of variation, the effects of the color of the container (straws) or the medium have not yet been evaluated. In this study, 13 ejaculates from different stallions were split into equal fractions, diluted either with Kenney or Equiplus extender, and subsequently packed into straws of five different colors. After storage at 4 °C for 24 h, the sperm were irradiated and different variables, including sperm motility, plasma membrane integrity, and mitochondrial membrane potential, were evaluated. Our results confirm that irradiation increases some motion characteristics and mitochondrial membrane potential without affecting sperm viability and demonstrate that the effects depend on the color of the straw and the extender used. Previous research has determined that irradiation of mammalian sperm with red light increases motility, mitochondrial activity, and fertilization capacity. In spite of this, no study has considered the potential influence of the color of the straw and the extender used. Therefore, this study tests the hypothesis that the response of mammalian sperm to red light is influenced by the color of the straw and the turbidity/composition of the extender. Using the horse as a model, 13 ejaculates from 13 stallions were split into two equal fractions, diluted with Kenney or Equiplus extender, and stored at 4 °C for 24 h. Thereafter, each diluted fraction was split into five equal aliquots and subsequently packed into 0.5-mL straws of red, blue, yellow, white, or transparent color. Straws were either nonirradiated (control) or irradiated with a light-dark-light pattern of 3-3-3 (i.e., light: 3 min, dark: 3 min; light: 3 min) prior to evaluating sperm motility, acrosome and ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Ministerio de Ciencia e Innovación RYC-2014-15581; Ministerio de Ciencia e Innovación AGL2017-88329-R; Agència de Gestió d'Ajuts Universitaris i de Recerca 2017-SGR-1229; Animals; Vol. 11 (january 2021); https://ddd.uab.cat/record/236224Test; urn:10.3390/ani11010122; urn:oai:ddd.uab.cat:236224; urn:pmcid:PMC7826721; urn:pmc-uid:7826721; urn:pmid:33429933; urn:oai:pubmedcentral.nih.gov:7826721; urn:oai:egreta.uab.cat:publications/8e3ac867-aa5f-4dd6-ba5b-a49d358b28d1 |
| الإتاحة: |
https://ddd.uab.cat/record/236224Test |
| حقوق: |
open access ; Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.94A08E00 |
| قاعدة البيانات: |
BASE |
