دورية أكاديمية
Monocyte-derived DC primed with TLR agonists secrete IL-12p70 in a CD40-dependent manner under hyperthermic conditions
| العنوان: | Monocyte-derived DC primed with TLR agonists secrete IL-12p70 in a CD40-dependent manner under hyperthermic conditions |
|---|---|
| المؤلفون: | Peng, Judy C., Hyde, Claire, Pai, Saparna, O'Sullivan, Brendan J., Nielsen, Lars K., Thomas, Ranjeny |
| المساهمون: | S. A. Rosenberg |
| بيانات النشر: | Lippincott Williams & Wilkins |
| سنة النشر: | 2006 |
| المجموعة: | The University of Queensland: UQ eSpace |
| مصطلحات موضوعية: | Oncology, Immunology, Medicine, Research & Experimental, human dendritic cells, hyperthermia, IL-12, CD40, Heat-shock-protein, Dendritic Cell Maturation, Whole-body Hyperthermia, Fever-range Hyperthermia, Serum-free Conditions, Nf-kappa-b, In-vivo, T-cells, Thermal-stress, Cd40 Ligation, 320202 Cellular Immunology, C1, 730102 Immune system and allergy, 1107 Immunology, 110704 Cellular Immunology |
| الوصف: | Fever is an evolutionarily conserved mechanism to improve survival during infection. Previous studies have shown that feverlike temperatures directly enhance the function of murine bone marrow-derived dendritic cells (DCs). In the present study, we examined the response of human monocyte-derived DC to 39.5 degrees C hyperthermia. When primed with toll-like receptor agonists or bacterial extract but not proinflammatory cytokines, hyperthermia specifically enhanced secretion of interleukin (IL)-12p70 by DC, without altering the secretion of IL-10, tumor necrosis factor a or IL-1 beta. These DC induced significantly higher levels of T-cell proliferation and interferon gamma production in assays of antigen presentation and MLR. Endogenous heat-sock protein 70 colocalized with CD40 in DC exposed to hyperthermic conditions. Recombinant CD40Fc fusion protein blocked the increase in IL-12p70 secretion by DC primed with bacterial extract and hyperthermia. Thus, DC primed with toll-like receptor-agonists respond to hyperthermia with increased IL-12p70 secretion, mediated by heat-shock protein binding and activation of CD40. The data have important applications for clinical immunotherapy and the mechanism of fever |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1524-9557 |
| العلاقة: | orcid:0000-0001-9773-2571; orcid:0000-0001-8191-3511; orcid:0000-0002-0518-8386 |
| الإتاحة: | https://doi.org/10.1097/01.cji.0000211308.82997.4eTest https://espace.library.uq.edu.au/view/UQ:119560Test |
| رقم الانضمام: | edsbas.9446FFF2 |
| قاعدة البيانات: | BASE |
| تدمد: | 15249557 |
|---|