دورية أكاديمية
Human adipose tissue H3K4me3 histone mark in adipogenic, lipid metabolism and inflammatory genes is positively associated with BMI and HOMA-IR.
| العنوان: | Human adipose tissue H3K4me3 histone mark in adipogenic, lipid metabolism and inflammatory genes is positively associated with BMI and HOMA-IR. |
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| المؤلفون: | Castellano-Castillo, Daniel, Denechaud, Pierre-Damien, Fajas, Lluis, Moreno-Indias, Isabel, Oliva-Olivera, Wilfredo, Tinahones, Francisco, Queipo-Ortuño, María Isabel, Cardona, Fernando |
| سنة النشر: | 2019 |
| المجموعة: | Sistema Sanitario Público de Andalucía (SSPA): Repositorio |
| مصطلحات موضوعية: | Adipogenesis, Adipose Tissue, Adult, Body Mass Index, Chromatin, DNA Methylation, Epigenesis, Genetic, Female, Gene Expression Regulation, Histones, Humans, Inflammation, Inflammation Mediators, Insulin Resistance, Lipid Metabolism, Male, Middle Aged, Promoter Regions |
| الوصف: | Adipose tissue is considered an important metabolic tissue, in charge of energy storage as well as being able to act in systemic homeostasis and inflammation. Epigenetics involves a series of factors that are important for gene regulation or for chromatin structure, mostly DNA methylation and histone-tail modifications, which can be modified by environmental conditions (nutrition, lifestyle, smoking…). Since metabolic diseases like obesity and diabetes are closely related to lifestyle and nutrition, epigenetic deregulation could play an important role in the onset of these diseases and vice versa. However, little is known about histone marks in human adipose tissue. In a previous work, we developed a protocol for chromatin immunoprecipitation (ChIP) of frozen human adipose tissue. By using this method, this study investigates, for the first time, the H3K4 trimethylation (H3K4me3) mark (open chromatin) on the promoter of several factors involved in adipogenesis, lipid metabolism and inflammation in visceral adipose tissue (VAT) from human subjects with different degrees of body mass index (BMI) and metabolic disease. VAT was collected and frozen at -80°C. 100 mg VAT samples were fixed in 0.5% formaldehyde and homogenized. After sonication, the sheared chromatin was immune-precipitated with an anti-H3K4me3 antibody linked to magnetic beads and purified. H3K4me3 enrichment was analyzed by qPCR for LEP, LPL, SREBF2, SCD1, PPARG, IL6, TNF and E2F1 promoters. mRNA extraction on the same samples was performed to quantify gene expression of these genes. H3K4me3 was enriched at the promoter of E2F1, LPL, SREBF2, SCD1, PPARG and IL6 in lean normoglycemic compared to morbid obese subjects with prediabetes. Accordingly H3K4me3 mark enrichment at E2F1, LPL, SREBF2, SCD1, PPARG and IL6 promoters was positively correlated with the BMI and the HOMA-IR. Regression analysis showed a strong relationship between the BMI with H3K4me3 at the promoter of E2F1 and LPL, and with mRNA levels of LEP and SCD. In the case of HOMA-IR, the ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | http://hdl.handle.net/10668/13797Test; PMC6453466; https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0215083&type=printableTest; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453466/pdfTest |
| DOI: | 10.1371/journal.pone.0215083 |
| DOI: | 10.1371/journal.pone.0215083&type=printable |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0215083Test http://hdl.handle.net/10668/13797Test https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0215083&type=printableTest https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453466/pdfTest |
| حقوق: | Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0Test/ ; open access |
| رقم الانضمام: | edsbas.943349B3 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0215083 |