دورية أكاديمية
SESSION 43: EMBRYOLOGY - EMBRYO CRYOPRESERVATION
| العنوان: | SESSION 43: EMBRYOLOGY - EMBRYO CRYOPRESERVATION |
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| المؤلفون: | Panagiotidis, Y., Kasapi, E., Goudakou, M., Papatheodorou, A., Pasadaki, T., Petousis, S., Nikolettos, N., Maroulis, G., Vanderzwalmen, P., Prapas, Y., Desai, N., Goldberg, J., Austin, C., Falcone, T., Nakagawa, K., Ojiro, Y., Takahashi, C., Sugiyama, R., Juen, H., Nishi, Y., Yoneda, Y., Shirai, A., Force, A., Schubert, B., Chomier, M., Sergeant, P., Lescaille, M., Rochigneux, S., Chassagnard, F., Rollet, J., Robert, F., Baucher, S., Sergeant, M.V., Ginon, J., Rodrigue, M., Nicollet, B., Barblett, H., Mehmet, D., Rebollar-Lazaro, I., Turner, S. |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2012 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Tuesday 3 July 2012 |
| الوصف: | Introduction: Vitrification is an increasingly popular method for the cryopreservation of human embryos in IVF programs worldwide. Nevertheless, due to the direct contact of samples to liquid nitrogen (LN2), safety issues are raised concerning cross contamination by bacteria or viruses during the cooling process or storage of embryos. In order to minimize such concerns, several closed vitrification systems have been proposed. In such systems, due to the encapsulation of the embryo carrier device in a protective cover, there is a decrease in the cooling rate of the samples. To compensate this, embryos should be maintained for an additional time in the cryoprotectants (CPs) solution. To avoid CPs toxicity, it has been proposed the use of CPs solutions of lower concentrations for the additional exposure time needed. The main objective of the study was to compare the same vitrification carrier device, the VitriSafe, in open (ultra-rapid vitrification) and closed (aseptic vitrification) conditions for the cryopreservation of human blastocysts. Materials and Methods: A prospective, randomized study was performed during 2009-2011 in a private assisted reproduction unit. Blastocysts of good to top quality, that were not eligible for a fresh embryo transfer, from 632 egg donation cycles, were randomized into group I (n = 309, open vitrification protocol) or group II (n = 323, closed vitrification protocol) by an allocation computer sequence. In total, 379 warming cycles were initiated, 185 originated from Group I and 194 from Group II. The VitriSafe (VitriMed, Austria) was used as embryo carrier device in both groups. All solutions used for vitrification and warming of blastocysts were equivalents to VitriFreeze and VitriThaw kit (Fertipro, Bernem, Belgium). In group I, solutions of ethylene glycol (EG) and dimethyl sulfoxide (DMSO) in concentrations of 10%-10% in the non-vitrification solution (NVS) and 20%-20% in the vitrification solution (VS) were used. In group II, there was an additional step of a 5%-5% NVS. ... |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://humrep.oxfordjournals.org/cgi/content/short/27/suppl_2/ii59-aTest; http://dx.doi.org/10.1093/humrep/27.s2.42Test |
| DOI: | 10.1093/humrep/27.s2.42 |
| الإتاحة: | https://doi.org/10.1093/humrep/27.s2.42Test http://humrep.oxfordjournals.org/cgi/content/short/27/suppl_2/ii59-aTest |
| حقوق: | Copyright (C) 2012, European Society of Human Reproduction and Embryology |
| رقم الانضمام: | edsbas.93F91DA8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/humrep/27.s2.42 |
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