دورية أكاديمية
A phage nucleus-associated RNA-binding protein is required for jumbo phage infection
| العنوان: | A phage nucleus-associated RNA-binding protein is required for jumbo phage infection |
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| المؤلفون: | Enustun, Eray, Armbruster, Emily G, Lee, Jina, Zhang, Sitao, Yee, Brian A, Malukhina, Kseniya, Gu, Yajie, Deep, Amar, Naritomi, Jack T, Liang, Qishan, Aigner, Stefan, Adler, Benjamin A, Cress, Brady F, Doudna, Jennifer A, Chaikeeratisak, Vorrapon, Cleveland, Don W, Ghassemian, Majid, Bintu, Bogdan, Yeo, Gene W, Pogliano, Joe, Corbett, Kevin D |
| المساهمون: | National Institutes of Health, Howard Hughes Medical Institute Emerging Pathogens Initiative, Howard Hughes Medical Institute, American Heart Association, U.S. Department of Energy, Biological and Environmental Research |
| المصدر: | Nucleic Acids Research ; volume 52, issue 8, page 4440-4455 ; ISSN 0305-1048 1362-4962 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2024 |
| الوصف: | Large-genome bacteriophages (jumbo phages) of the proposed family Chimalliviridae assemble a nucleus-like compartment bounded by a protein shell that protects the replicating phage genome from host-encoded restriction enzymes and DNA-targeting CRISPR-Cas nucleases. While the nuclear shell provides broad protection against host nucleases, it necessitates transport of mRNA out of the nucleus-like compartment for translation by host ribosomes, and transport of specific proteins into the nucleus-like compartment to support DNA replication and mRNA transcription. Here, we identify a conserved phage nuclear shell-associated protein that we term Chimallin C (ChmC), which adopts a nucleic acid-binding fold, binds RNA with high affinity in vitro, and binds phage mRNAs in infected cells. ChmC also forms phase-separated condensates with RNA in vitro. Targeted knockdown of ChmC using mRNA-targeting dCas13d results in accumulation of phage-encoded mRNAs in the phage nucleus, reduces phage protein production, and compromises virion assembly. Taken together, our data show that the conserved ChmC protein plays crucial roles in the viral life cycle, potentially by facilitating phage mRNA translocation through the nuclear shell to promote protein production and virion development. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/nar/gkae216 |
| الإتاحة: | https://doi.org/10.1093/nar/gkae216Test https://academic.oup.com/nar/article-pdf/52/8/4440/57446571/gkae216.pdfTest |
| حقوق: | https://creativecommons.org/licenses/by-nc/4.0Test/ |
| رقم الانضمام: | edsbas.933910BE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/gkae216 |
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