دورية أكاديمية
Conservation Analysis of Dengue Virus T-cell Epitope-Based Vaccine Candidates Using Peptide Block Entropy
| العنوان: | Conservation Analysis of Dengue Virus T-cell Epitope-Based Vaccine Candidates Using Peptide Block Entropy |
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| المؤلفون: | Olsen, Lars Rønn, Zhang, Guang Lan, Keskin, Derin Benerci, Reinherz, Ellis Leonard, Brusic, Vladimir |
| بيانات النشر: | Frontiers Research Foundation |
| سنة النشر: | 2011 |
| المجموعة: | Harvard University: DASH - Digital Access to Scholarship at Harvard |
| مصطلحات موضوعية: | antigenic diversity, epitope-based vaccines, immunoinformatics, polyvalent vaccines, reverse vaccinology, vaccine informatics |
| الوصف: | Broad coverage of the pathogen population is particularly important when designing CD8+ T-cell epitope vaccines against viral pathogens. Traditional approaches are based on combinations of highly conserved T-cell epitopes. Peptide block entropy analysis is a novel approach for assembling sets of broadly covering antigens. Since T-cell epitopes are recognized as peptides rather than individual residues, this method is based on calculating the information content of blocks of peptides from a multiple sequence alignment of homologous proteins rather than using the information content of individual residues. The block entropy analysis provides broad coverage of variant antigens. We applied the block entropy analysis method to the proteomes of the four serotypes of dengue virus (DENV) and found 1,551 blocks of 9-mer peptides, which cover 99% of available sequences with five or fewer unique peptides. In contrast, the benchmark study by Khan et al. (2008) resulted in 165 conserved 9-mer peptides. Many of the conserved blocks are located consecutively in the proteins. Connecting these blocks resulted in 78 conserved regions. Of the 1551 blocks of 9-mer peptides 110 comprised predicted HLA binder sets. In total, 457 subunit peptides that encompass the diversity of all sequenced DENV strains of which 333 are T-cell epitope candidates. ; Version of Record |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1664-3224 |
| العلاقة: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3341948/pdfTest/; Frontiers in Immunology; Olsen, Lars Rønn, Guang Lan Zhang, Derin B. Keskin, Ellis L. Reinherz, and Vladimir Brusic. 2011. Conservation analysis of dengue virus T-cell epitope-based vaccine candidates using peptide block entropy. Frontiers in Immunology 2:69.; http://nrs.harvard.edu/urn-3:HUL.InstRepos:10364593Test |
| DOI: | 10.3389/fimmu.2011.00069 |
| الإتاحة: | https://doi.org/10.3389/fimmu.2011.00069Test http://nrs.harvard.edu/urn-3:HUL.InstRepos:10364593Test |
| رقم الانضمام: | edsbas.931746B6 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 16643224 |
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| DOI: | 10.3389/fimmu.2011.00069 |