التفاصيل البيبلوغرافية
| العنوان: |
The accumulation of erythrocytes quantified and visualized by Glycophorin C in carotid atherosclerotic plaque reflects intraplaque hemorrhage and pre-procedural neurological symptoms |
| المؤلفون: |
Mekke, Joost M, Sakkers, Tim R, Verwer, Maarten C, van den Dungen, Noortje A M, Song, Yipei, Miller, Clint L, Finn, Aloke V, Pasterkamp, Gerard, Mokry, Michal, den Ruijter, Hester M, Vink, Aryan, de Kleijn, Dominique P V, de Borst, Gert J, Haitjema, Saskia, van der Laan, Sander W |
| المساهمون: |
Zorgeenheid Vaatchirurgie Medisch, Experimentele Afd. Cardiologie 1, Circulatory Health, CDL Research analisten, Cancer, CDL Onderzoek Pasterkamp, Centraal Diagnostisch Laboratorium, Child Health, Onderzoek Vrouw Hart & Vaatziekten, Pathologie Pathologen staf, Infection & Immunity, Regenerative Medicine and Stem Cells, CDL Cluster Speciële Diagnostiek |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
Calcinosis/pathology, Carotid Arteries/pathology, Carotid Stenosis/pathology, Collagen, Erythrocyte Membrane/pathology, Female, Glycophorins, Hemorrhage/pathology, Humans, Lipids, Magnetic Resonance Imaging, Male, Plaque, Atherosclerotic/pathology, General, Journal Article, Research Support, Non-U.S. Gov't |
| الوصف: |
The accumulation of erythrocyte membranes within an atherosclerotic plaque may contribute to the deposition of free cholesterol and thereby the enlargement of the necrotic core. Erythrocyte membranes can be visualized and quantified in the plaque by immunostaining for the erythrocyte marker glycophorin C. Hence, we theorized that the accumulation of erythrocytes quantified by glycophorin C could function as a marker for plaque vulnerability, possibly reflecting intraplaque hemorrhage (IPH), and offering predictive value for pre-procedural neurological symptoms. We employed the CellProfiler-integrated slideToolKit workflow to visualize and quantify glycophorin C, defined as the total plaque area that is positive for glycophorin C, in single slides of culprit lesions obtained from the Athero-Express Biobank of 1819 consecutive asymptomatic and symptomatic patients who underwent carotid endarterectomy. Our assessment included the evaluation of various parameters such as lipid core, calcifications, collagen content, SMC content, and macrophage burden. These parameters were evaluated using a semi-quantitative scoring method, and the resulting data was dichotomized as predefined criteria into categories of no/minor or moderate/heavy staining. In addition, the presence or absence of IPH was also scored. The prevalence of IPH and pre-procedural neurological symptoms were 62.4% and 87.1%, respectively. The amount of glycophorin staining was significantly higher in samples from men compared to samples of women (median 7.15 (IQR:3.37, 13.41) versus median 4.06 (IQR:1.98, 8.32), p < 0.001). Glycophorin C was associated with IPH adjusted for clinical confounders (OR 1.90; 95% CI 1.63, 2.21; p = < 0.001). Glycophorin C was significantly associated with ipsilateral pre-procedural neurological symptoms (OR:1.27, 95%CI:1.06-1.41, p = 0.005). Sex-stratified analysis, showed that this was also the case for men (OR 1.37; 95%CI 1.12, 1.69; p = 0.003), but not for women (OR 1.15; 95%CI 0.77, 1.73; p = 0.27). Glycophorin C was ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
2045-2322 |
| العلاقة: |
https://dspace.library.uu.nl/handle/1874/450219Test |
| الإتاحة: |
https://dspace.library.uu.nl/handle/1874/450219Test |
| حقوق: |
info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: |
edsbas.92EABE99 |
| قاعدة البيانات: |
BASE |
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