التفاصيل البيبلوغرافية
| العنوان: |
CD38 marks the exhausted CD8+ tissue-resident memory T cells in hepatocellular carcinoma |
| المؤلفون: |
Reolo, Marie J. Y., Otsuka, Masayuki, Seow, Justine Jia Wen, Lee, Joycelyn, Lee, Yun Hua, Nguyen, Phuong H. D., Lim, Chun Jye, Wasser, Martin, Chua, Camillus, Lim, Tony K. H., Leow, Wei Qiang, Chung, Alexander, Goh, Brian K. P., Chow, Pierce K. H., DasGupta, Ramanuj, Yeong, Joe Poh Sheng, Chew, Valerie |
| المصدر: |
Frontiers in Immunology ; volume 14 ; ISSN 1664-3224 |
| بيانات النشر: |
Frontiers Media SA |
| سنة النشر: |
2023 |
| المجموعة: |
Frontiers (Publisher - via CrossRef) |
| مصطلحات موضوعية: |
Immunology, Immunology and Allergy |
| الوصف: |
Introduction Despite recent advances in immunotherapy for hepatocellular carcinoma (HCC), the overall modest response rate underscores the need for a better understanding of the tumor microenvironment (TME) of HCC. We have previously shown that CD38 is widely expressed on tumor-infiltrating leukocytes (TILs), predominantly on CD3 + T cells and monocytes. However, its specific role in the HCC TME remains unclear. Methods In this current study, we used cytometry time-of-flight (CyTOF), bulk RNA sequencing on sorted T cells, and single-cell RNA (scRNA) sequencing to interrogate expression of CD38 and its correlation with T cell exhaustion in HCC samples. We also employed multiplex immunohistochemistry (mIHC) for validating our findings. Results From CyTOF analysis, we compared the immune composition of CD38-expressing leukocytes in TILs, non-tumor tissue-infiltrating leukocytes (NIL), and peripheral blood mononuclear cells (PBMC). We identified CD8 + T cells as the dominant CD38-expressing TILs and found that CD38 expression was significantly higher in CD8 + T RM in TILs than in NILs. Furthermore, through transcriptomic analysis on sorted CD8 + T RM from HCC tumors, we observed a higher expression of CD38 along with T cell exhaustion genes, including PDCD1 and CTLA4, compared to the circulating memory CD8 T cells from PBMC. This was validated by scRNA sequencing that revealed co-expression of CD38 with PDCD1, CTLA4, and ITGAE (CD103) in T cells from HCC tumors. The protein co-expression of CD38 and PD-1 on CD8 + T cells was further demonstrated by mIHC on HCC FFPE tissues, marking CD38 as a T cell co-exhaustion marker in HCC. Lastly, the higher proportions of CD38 + PD-1 + CD8 + T cells and CD38 + PD-1 + T RM were significantly associated with the higher histopathological grades of HCC, indicating its role in the aggressiveness of the disease. Conclusion Taken together, the concurrent expression of CD38 with exhaustion markers on CD8 + T RM underpins its role as a key marker of T cell exhaustion and a potential ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| DOI: |
10.3389/fimmu.2023.1182016 |
| DOI: |
10.3389/fimmu.2023.1182016/full |
| الإتاحة: |
https://doi.org/10.3389/fimmu.2023.1182016Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.92C20D1F |
| قاعدة البيانات: |
BASE |
