دورية أكاديمية
The genome of a bunyavirus cannot be defined at the level of the viral particle but only at the scale of the viral population
| العنوان: | The genome of a bunyavirus cannot be defined at the level of the viral particle but only at the scale of the viral population |
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| المؤلفون: | Yvon, Michel, German, Thomas, Ullman, Diane, Dasgupta, Ranjit, Parker, Maxwell, Ben-Mahmoud, Sulley, Verdin, Eric, Gognalons, Patrick, Ancelin, Aurélie, Laï Kee Him, Joséphine, Girard, Justine, Vernerey, Marie-Stéphanie, Fernandez, Emmanuel, Filloux, Denis, Roumagnac, Philippe, Bron, Patrick, Michalakis, Yannis, Blanc, Stéphane |
| المساهمون: | Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), University of Wisconsin-Madison, University of California (UC), Unité de Pathologie Végétale (PV), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de Biologie Structurale Montpellier (CBS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD France-Sud )-Université de Montpellier (UM), We are deeply thankful for the contribution of our colleague T.L.G. and for his timeless imprint on this work. We are grateful to MUSE Univ Montpellier for funding this work (project BLANC-MUSE2020-Multivir). M.Y., E.V., P.G., M.-S.V., and S.B. acknowledge support from INRAE department SPE, Y. Michalakis from CNRS and IRD, P.B., J.L.K.H., A.A., and J.G. from INSERM, E.F., D.F., and P.R. from CIRAD. D.E.U. acknowledges support from the Fulbright Scholar Program and sabbatical leave from University of California Davis. T.L.G. acknowledges funding for this work was provided in part by the USDA-ARS Floriculture and Nursery Research Initiative project# 58-6034-5-029. We thank Jean-Marc Leininger and Norma Ordaz for technical support in providing virus isolate. |
| المصدر: | ISSN: 0027-8424. |
| بيانات النشر: | HAL CCSD National Academy of Sciences |
| سنة النشر: | 2023 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | Bunyavirales, genome formula, genome packaging, multipartite virus, orthotospovirus, MESH: Orthobunyavirus, MESH: RNA, Viral, MESH: Tospovirus, MESH: Genome, MESH: Virion, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.GEN]Life Sciences [q-bio]/Genetics |
| الوصف: | International audience ; Bunyaviruses are enveloped negative or ambisense single-stranded RNA viruses with a genome divided into several segments. The canonical view depicts each viral particle packaging one copy of each genomic segment in one polarity named the viral strand. Several opposing observations revealed nonequal ratios of the segments, uneven number of segments per virion, and even packaging of viral complementary strands. Unfortunately, these observations result from studies often addressing other questions, on distinct viral species, and not using accurate quantitative methods. Hence, what RNA segments and strands are packaged as the genome of any bunyavirus remains largely ambiguous. We addressed this issue by first investigating the virion size distribution and RNA content in populations of the tomato spotted wilt virus (TSWV) using microscopy and tomography. These revealed heterogeneity in viral particle volume and amount of RNA content, with a surprising lack of correlation between the two. Then, the ratios of all genomic segments and strands were established using RNA sequencing and qRT-PCR. Within virions, both plus and minus strands (but no mRNA) are packaged for each of the three L, M, and S segments, in reproducible nonequimolar proportions determined by those in total cell extracts. These results show that virions differ in their genomic content but together build up a highly reproducible genetic composition of the viral population. This resembles the genome formula described for multipartite viruses, with which some species of the order Bunyavirales may share some aspects of the way of life, particularly emerging properties at a supravirion scale. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-01-157398-4 0-01-157398-8 |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/37983500; hal-04313387; https://hal.science/hal-04313387Test; https://hal.science/hal-04313387/documentTest; https://hal.science/hal-04313387/file/2023-09412-R1-HALV2.pdfTest; PUBMED: 37983500; WOS: 001157398800006 |
| DOI: | 10.1073/pnas.2309412120 |
| الإتاحة: | https://doi.org/10.1073/pnas.2309412120Test https://hal.science/hal-04313387Test https://hal.science/hal-04313387/documentTest https://hal.science/hal-04313387/file/2023-09412-R1-HALV2.pdfTest |
| رقم الانضمام: | edsbas.91F137F8 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780011573984 0011573988 |
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| DOI: | 10.1073/pnas.2309412120 |