دورية أكاديمية
Transcriptomic diversity in human medullary thymic epithelial cells
| العنوان: | Transcriptomic diversity in human medullary thymic epithelial cells |
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| المؤلفون: | Carter, Jason A., Strömich, Léonie, Peacey, Matthew, Chapin, Sarah R., Velten, Lars, Steinmetz, Lars M., Brors, Benedikt, Pinto, Sheena, Meyer, Hannah V. |
| المصدر: | Nature Communications ; volume 13, issue 1 ; ISSN 2041-1723 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | General Physics and Astronomy, General Biochemistry, Genetics and Molecular Biology, General Chemistry, Multidisciplinary |
| الوصف: | The induction of central T cell tolerance in the thymus depends on the presentation of peripheral self-epitopes by medullary thymic epithelial cells (mTECs). This promiscuous gene expression (pGE) drives mTEC transcriptomic diversity, with non-canonical transcript initiation, alternative splicing, and expression of endogenous retroelements (EREs) representing important but incompletely understood contributors. Here we map the expression of genome-wide transcripts in immature and mature human mTECs using high-throughput 5’ cap and RNA sequencing. Both mTEC populations show high splicing entropy, potentially driven by the expression of peripheral splicing factors. During mTEC maturation, rates of global transcript mis-initiation increase and EREs enriched in long terminal repeat retrotransposons are up-regulated, the latter often found in proximity to differentially expressed genes. As a resource, we provide an interactive public interface for exploring mTEC transcriptomic diversity. Our findings therefore help construct a map of transcriptomic diversity in the healthy human thymus and may ultimately facilitate the identification of those epitopes which contribute to autoimmunity and immune recognition of tumor antigens. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41467-022-31750-1 |
| الإتاحة: | https://doi.org/10.1038/s41467-022-31750-1Test https://www.nature.com/articles/s41467-022-31750-1.pdfTest https://www.nature.com/articles/s41467-022-31750-1Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.91BAA31E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-022-31750-1 |
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