دورية أكاديمية
Transcriptional programming of immunoregulatory responses in human Langerhans cells
| العنوان: | Transcriptional programming of immunoregulatory responses in human Langerhans cells |
|---|---|
| المؤلفون: | Davies, James, Sirvent, Sofia, Vallejo, Andres F., Clayton, Kalum, Douilhet, Gemma, Keeler, Patrick S., West, Jonathan, Ardern-Jones, Michael, MacArthur, Ben D., Singh, Harinder, Polak, Marta E. |
| سنة النشر: | 2022 |
| المجموعة: | University of Southampton: e-Prints Soton |
| الوصف: | Human epidermal Langerhans cells (LCs) maintain immune homeostasis in the skin. To examine transcriptional programming of human primary LCs during homeostasis, we performed scRNA-seq analysis of LCs before and after migration from the epidermis, coupled with functional assessment of their regulatory T cell priming capabilities. The analysis revealed that steady-state LCs exist in a continuum of maturation states and upregulate antigen presentation genes along with an immunoregulatory module including the genes IDO1, LGALS1, LAMTOR1, IL4I, upon their migration. The migration-induced transition in genomic state is accompanied by the ability of LCs to more efficiently prime regulatory T cell responses in co-culture assays. Computational analyses of the scRNAseq datasets using SCENIC and Partial Information Decomposition in Context identified a set of migration-induced transcription factors including IRF4, KLF6 and RelB as key nodes within a immunoregulatory gene regulatory network. These findings support a model in which efficient priming of immunoregulatory responses by LCs is dependent on coordinated upregulation of a migration-coupled maturation program with a immunoregulation-promoting genomic module. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://eprints.soton.ac.uk/475121/1/fimmu_13_892254.pdfTest; Davies, James, Sirvent, Sofia, Vallejo, Andres F., Clayton, Kalum, Douilhet, Gemma, Keeler, Patrick S., West, Jonathan, Ardern-Jones, Michael, MacArthur, Ben D., Singh, Harinder and Polak, Marta E. (2022) Transcriptional programming of immunoregulatory responses in human Langerhans cells. Frontiers in Immunology, 13, [892254]. (doi:10.3389/fimmu.2022.892254 ). |
| الإتاحة: | https://doi.org/10.3389/fimmu.2022.892254Test https://eprints.soton.ac.uk/475121Test/ https://eprints.soton.ac.uk/475121/1/fimmu_13_892254.pdfTest |
| حقوق: | cc_by_4 |
| رقم الانضمام: | edsbas.91A3C35D |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |