دورية أكاديمية
Identification and comparison of microRNAs from skeletal muscle and adipose tissues from two porcine breeds
| العنوان: | Identification and comparison of microRNAs from skeletal muscle and adipose tissues from two porcine breeds |
|---|---|
| المؤلفون: | Li, Hong-Yi, Xi, Qian-Yun, Xiong, Yuan-Yan, Liu, Xiao-Long, Cheng, Xiao, Shu, Gang, Wang, Song-Bo, Wang, Li-Na, Gao, Ping, Zhu, Xiao-Tong, Jiang, Qing-Yan, Yuan, Li, Zhang, Yong-Liang, 袁立 |
| المصدر: | http://dx.doi.org/10.1111/j.1365-2052.2012.02332.xTest. |
| بيانات النشر: | ANIM GENET |
| سنة النشر: | 2012 |
| المجموعة: | Xiamen University Institutional Repository |
| مصطلحات موضوعية: | ADIPOCYTE DIFFERENTIATION, SIGNALING PATHWAY, PPAR-GAMMA, EXPRESSION, RNA, PROLIFERATION, ADIPOGENESIS, BIOGENESIS, REGULATOR, GENOMICS |
| الوصف: | Chinese Key Basic Plan [2009CB941600]; National Natural Science Foundation of China [31072105, u0731004]; Guangdong Natural Science Foundation [10451064201005460]; national Key project [2009ZX0890-024B] ; MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that negatively regulate gene expression at the post-transcriptional level. Although an increasing number of porcine miRNAs recently have been identified, research has yet to identify the full repertoire of miRNAs in pig skeletal and adipose tissues and their differences between breeds. We extracted small RNA from skeletal muscle and adipose tissues of Landrace and Lantang pigs, and the expression of a total of 184 known porcine miRNAs (113 from Solexa sequencing and 171 from miRNA chip hybridization) as well as 521 novel miRNA candidates was detected. Moreover, 20 miRNAs were selected randomly from the 184 miRNAs and analysed by quantitative real-time PCR to confirm the aforementioned results. In the skeletal muscle tissues, 21 miRNAs were up-regulated in Lantang and another 33 were highly expressed in Landrace pigs. In the adipose tissues, 25 miRNAs were down-regulated in Lantang and another 23 were lowly expressed in Landrace pigs. miRNA divergence between tissues was also detected in this study. Ten miRNAs were highly expressed in the skeletal muscle tissue in comparison with adipose tissue, and another 10 miRNAs exhibited the opposite expression profile. To investigate the regulatory mechanism of the miRNAs in muscle and adipose tissues, the 10 miRNAs with the most divergent expression profiles were functionally categorized using the Kyoto Encyclopedia of Genes and Genomes database. Most of the miRNAs strongly corresponded to myogenesis and adipogenesis processes. In addition, 84 of the 521 miRNA candidates were potentially porcine-specific miRNAs. This study adds new valuable information to comparative miRNA profiles of skeletal muscle and adipose tissues in porcine species. The great diversity of miRNA composition and expression levels ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | ANIMAL GENETICS, 2012,43(6):704-713; WOS:000310064000007; http://dspace.xmu.edu.cn/handle/2288/90460Test |
| الإتاحة: | https://doi.org/10.1111/j.1365-2052.2012.02332.xTest http://dspace.xmu.edu.cn/handle/2288/90460Test |
| رقم الانضمام: | edsbas.900BA871 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |