دورية أكاديمية
PNA-based microRNA detection methodologies
| العنوان: | PNA-based microRNA detection methodologies |
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| المؤلفون: | Cadoni, Enrico, Manicardi, Alex, Madder, Annemieke |
| المصدر: | MOLECULES ; ISSN: 1420-3049 |
| سنة النشر: | 2020 |
| المجموعة: | Ghent University Academic Bibliography |
| مصطلحات موضوعية: | Chemistry, PEPTIDE-NUCLEIC-ACID, GOLD NANOPARTICLES, GRAPHENE OXIDE, TEMPLATED, REACTIONS, CANCER, GENE, AMPLIFICATION, DNA, MIRNA, HYBRIDIZATION, peptide nucleic acid (PNA), microRNA, fluorescence, templated reactions, nanoparticles, light-triggered, electrochemical biosensors, colorimetric, detection |
| الوصف: | MicroRNAs (miRNAs or miRs) are small noncoding RNAs involved in the fine regulation of post-transcriptional processes in the cell. The physiological levels of these short (20-22-mer) oligonucleotides are important for the homeostasis of the organism, and therefore dysregulation can lead to the onset of cancer and other pathologies. Their importance as biomarkers is constantly growing and, in this context, detection methods based on the hybridization to peptide nucleic acids (PNAs) are gaining their place in the spotlight. After a brief overview of their biogenesis, this review will discuss the significance of targeting miR, providing a wide range of PNA-based approaches to detect them at biologically significant concentrations, based on electrochemical, fluorescence and colorimetric assays. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://biblio.ugent.be/publication/8666907Test; http://hdl.handle.net/1854/LU-8666907Test; http://dx.doi.org/10.3390/molecules25061296Test; https://biblio.ugent.be/publication/8666907/file/8666908Test |
| DOI: | 10.3390/molecules25061296 |
| الإتاحة: | https://doi.org/10.3390/molecules25061296Test https://biblio.ugent.be/publication/8666907Test http://hdl.handle.net/1854/LU-8666907Test https://biblio.ugent.be/publication/8666907/file/8666908Test |
| حقوق: | Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.8E4EF90A |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/molecules25061296 |
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