دورية أكاديمية
Identification and Characterization of a Ribose 2 '-O-Methyltransferase Encoded by the Ronivirus Branch of Nidovirales
| العنوان: | Identification and Characterization of a Ribose 2 '-O-Methyltransferase Encoded by the Ronivirus Branch of Nidovirales |
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| المؤلفون: | Zeng, C., Wu, A.D., Wang, Y., Xu, S., Tang, Y.K., Jin, X., Wang, S.L., Qin, L., Sun, Y., Fan, C.P., Snijder, E.J., Neuman, B.W., Chen, Y., Ahola, T., Guo, D.Y. |
| المصدر: | Journal of Virology |
| سنة النشر: | 2016 |
| المجموعة: | Leiden Repository (Leiden University) |
| الوصف: | Methylation of the 5'-cap structure of viral RNAs plays important roles in genome replication and evasion of innate recognition of viral RNAs by cellular sensors. It is known that coronavirus nsp14 acts as an N7-(guanine)-methyltransferase (MTase) and nsp16 as a 2'-O-MTase, which are involved in the modification of RNA cap structure. However, these enzymatic activities have not been shown for any other nidoviruses beyond coronaviruses in the order Nidovirales. In this study, we identified a 2'-O-methyltransferase encoded by ronivirus that shows common and unique features in comparison with that of coronaviruses. Ronivirus 2'-O-MTase does not need a protein cofactor for MTase activity, whereas coronavirus nsp16 needs the stimulating factor nsp10 for its full activity. The conserved K-D-K-E catalytic tetrad is identified in ronivirus 2'-O-MTase. These results extend our understanding of nidovirus RNA capping and methylation beyond coronaviruses and also strengthen the evolutionary and functional links between roniviruses and coronaviruses. ; Molecular basis of virus replication, viral pathogenesis and antiviral strategies |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | lumc-id: 3408145; https://hdl.handle.net/1887/99463Test |
| DOI: | 10.1128/JVI.00658-16 |
| الإتاحة: | https://doi.org/10.1128/JVI.00658-16Test https://hdl.handle.net/1887/99463Test |
| رقم الانضمام: | edsbas.8DD54C72 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/JVI.00658-16 |
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