دورية أكاديمية
Identification of a Profile of Neutrophil-Derived Granule Proteins in the Surface of Gold Nanoparticles after Their Interaction with Human Breast Cancer Sera
| العنوان: | Identification of a Profile of Neutrophil-Derived Granule Proteins in the Surface of Gold Nanoparticles after Their Interaction with Human Breast Cancer Sera |
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| المؤلفون: | Chantada Vázquez, María Pilar, García Vence, María, Vázquez Estévez, Sergio, Bravo López, Susana Belén, Núñez González, Cristina |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | CHUS, HULA, IDIS |
| الوصف: | It is well known that the interaction of a nanomaterial with a biological fluid leads to the formation of a protein corona (PC) surrounding the nanomaterial. Using standard blood analyses, alterations in protein patterns are difficult to detect. PC acts as a "nano-concentrator" of serum proteins with affinity for nanoparticles' surface. Consequently, characterization of PC could allow detection of otherwise undetectable changes in protein concentration at an early stage of a disease, such as breast cancer (BC). Here, we employed gold nanoparticles (AuNPsdiameter: 10.02 +/- 0.91 nm) as an enrichment platform to analyze the human serum proteome of BC patients (n = 42) and healthy controls (n = 42). Importantly, the analysis of the PC formed around AuNPs after their interaction with serum samples of BC patients showed a profile of proteins that could differentiate breast cancer patients from healthy controls. These proteins developed a significant role in the immune and/or innate immune system, some of them being neutrophil-derived granule proteins. The analysis of the PC also revealed serum proteome alterations at the subtype level. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 2079-4991 |
| العلاقة: | https://www.ncbi.nlm.nih.gov/pubmed/32586001Test; http://hdl.handle.net/20.500.11940/16471Test; 38974 |
| DOI: | 10.3390/nano10061223 |
| الإتاحة: | https://doi.org/20.500.11940/16471Test https://doi.org/10.3390/nano10061223Test https://hdl.handle.net/20.500.11940/16471Test https://www.ncbi.nlm.nih.gov/pubmed/32586001Test |
| حقوق: | Atribución 4.0 Internacional ; http://creativecommons.org/licenses/by/4.0Test/ ; openAccess |
| رقم الانضمام: | edsbas.8C2F5822 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20794991 |
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| DOI: | 10.3390/nano10061223 |