دورية أكاديمية
A PCR, qPCR, and LAMP Toolkit for the Detection of the Wheat Blast Pathogen in Seeds
| العنوان: | A PCR, qPCR, and LAMP Toolkit for the Detection of the Wheat Blast Pathogen in Seeds |
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| المؤلفون: | Thierry, Maud, Chatet, Axel, Fournier, Elisabeth, Tharreau, Didier, Ioos, Renaud, R. |
| المساهمون: | Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| المصدر: | ISSN: 2223-7747 ; Plants ; https://hal.inrae.fr/hal-02622819Test ; Plants, 2020, 9 (2), pp.277. ⟨10.3390/plants9020277⟩. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | diagnostic, pyricularia oryzae, seed testing, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
| الوصف: | International audience ; Wheat blast is a devastating disease caused by the pathogenic fungus Pyricularia oryzae. Wheat blast first emerged in South America before more recently reaching Bangladesh. Even though the pathogen can spread locally by air-dispersed spores, long-distance spread is likely to occur via infected wheat seed or grain. Wheat blast epidemics are caused by a genetic lineage of the fungus, called the Triticum lineage, only differing from the other P. oryzae lineages by less than 1% genetic divergence. In order to prevent further spread of this pathogen to other wheat-growing areas in the world, sensitive and specific detection tools are needed to test for contamination of traded seed lots by the P. oryzae Triticum lineage. In this study, we adopted a comparative genomics approach to identify new loci specific to the P. oryzae Triticum lineage and used them to design a set of new markers that can be used in conventional polymerase chain reaction (PCR), real-time PCR, or loop-mediated isothermal amplification (LAMP) for the detection of the pathogen, with improved inclusivity and specificity compared to currently available tests. A preliminary biological enrichment step of the seeds was shown to improve the sensitivity of the tests, which enabled the detection of the target at an infection rate as low as 0.25%. Combined with others, this new toolkit may be particularly beneficial in preventing the trade of contaminated seeds and in limiting the spread of the disease |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/32098075; hal-02622819; https://hal.inrae.fr/hal-02622819Test; https://hal.inrae.fr/hal-02622819/documentTest; https://hal.inrae.fr/hal-02622819/file/Publis-bgpi-003_thierry_PCR_1.pdfTest; PRODINRA: 496295; PUBMED: 32098075; WOS: 000519248200038 |
| DOI: | 10.3390/plants9020277 |
| الإتاحة: | https://doi.org/10.3390/plants9020277Test https://hal.inrae.fr/hal-02622819Test https://hal.inrae.fr/hal-02622819/documentTest https://hal.inrae.fr/hal-02622819/file/Publis-bgpi-003_thierry_PCR_1.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.8BB6AC07 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/plants9020277 |
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