دورية أكاديمية
Svep1 stabilises developmental vascular anastomosis in reduced flow conditions
| العنوان: | Svep1 stabilises developmental vascular anastomosis in reduced flow conditions |
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| المؤلفون: | Coxam, B., Collins, R.T., Hußmann, M., Huisman, Y., Meier, K., Jung, S., Bartels-Klein, E., Szymborska, A., Finotto, L., Helker, C.S.M., Stainier, D.Y.R., Schulte-Merker, S., Gerhardt, H. |
| بيانات النشر: | Company of Biologists |
| سنة النشر: | 2022 |
| المجموعة: | Max-Delbrueck-Center for Molecular Medicine, Berlin: MDC Repository |
| مصطلحات موضوعية: | Cardiovascular and Metabolic Diseases, Topic 3: Integrative Biomedicine |
| الوصف: | Molecular mechanisms controlling the formation, stabilization and maintenance of blood vessel connections remain poorly defined. Here we identify blood flow and the large extracellular protein Svep1 as co-modulators of vessel anastomosis during developmental angiogenesis in zebrafish embryos. Both loss of Svep1 and blood flow reduction contribute to defective anastomosis of intersegmental vessels. The reduced formation and lumenisation of the dorsal longitudinal anastomotic vessel (DLAV) is associated with a compensatory increase in Vegfa/Vegfr pERK signalling, concomittant expansion of apelin-positive tip cells, but reduced expression of klf2. Experimentally, further increasing Vegfa/Vegfr signalling can rescue the DLAV formation and lumenisation defects, while its inhibition dramatically exacerbates the loss of connectivity. Mechanistically, our results suggest that flow and Svep1 co-regulate the stabilization of vascular connections, in part by modulating the Vegfa/Vegfr signalling pathway. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://edoc.mdc-berlin.de/21369/1/21369oa.pdfTest; Svep1 stabilises developmental vascular anastomosis in reduced flow conditions. Coxam, B. and Collins, R.T. and Hußmann, M. and Huisman, Y. and Meier, K. and Jung, S. and Bartels-Klein, E. and Szymborska, A. and Finotto, L. and Helker, C.S.M. and Stainier, D.Y.R. and Schulte-Merker, S. and Gerhardt, H. Development 149 (6): dev199858. 24 March 2022 |
| DOI: | 10.1242/dev.199858 |
| الإتاحة: | https://doi.org/10.1242/dev.199858Test http://edoc.mdc-berlin.de/21369Test/ https://edoc.mdc-berlin.de/21369Test/ http://edoc.mdc-berlin.de/21369/1/21369oa.pdfTest |
| حقوق: | cc_by_4 |
| رقم الانضمام: | edsbas.8B9D2518 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1242/dev.199858 |
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