دورية أكاديمية
Quantitative Flow Cytometric Evaluation of Oxidative Stress and Mitochondrial Impairment in RAW 264.7 Macrophages after Exposure to Pristine, Acid Functionalized, or Annealed Carbon Nanotubes
| العنوان: | Quantitative Flow Cytometric Evaluation of Oxidative Stress and Mitochondrial Impairment in RAW 264.7 Macrophages after Exposure to Pristine, Acid Functionalized, or Annealed Carbon Nanotubes |
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| المؤلفون: | Sabido, Odile, Figarol, Agathe, Klein, Jean-Philippe, Bin, Valérie, Forest, Valérie, Pourchez, Jérémie, Fubini, Bice, Cottier, Michèle, Tomatis, Maura, Boudard, Delphine |
| المساهمون: | Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon, Centre commun de Cytométrie en Flux (CCCF), Université Jean Monnet - Saint-Étienne (UJM), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté COMUE (UBFC)-Université Bourgogne Franche-Comté COMUE (UBFC), Università degli studi di Torino = University of Turin (UNITO) |
| المصدر: | ISSN: 2079-4991. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2020 |
| المجموعة: | Université Jean Monnet – Saint-Etienne: HAL |
| مصطلحات موضوعية: | +33-477421441 (O.S.), carbon nanotubes, acid functionalization, annealing treatment, oxidative stress, mitochondrial membrane potential collapse, cytotoxicity, chromatin decondensation, scavenging capacity, flow cytometry and spin trapping in-cell free system, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Conventional nanotoxicological assays are subjected to various interferences with nanoparticles and especially carbon nanotubes. A multiparametric flow cytometry (FCM) methodology was developed here as an alternative to quantify oxidative stress, mitochondrial impairment, and later cytotoxic and genotoxic events. The experiments were conducted on RAW264.7 macrophages, exposed for 90 min or 24 h-exposure with three types of multiwalled carbon nanotubes (MWCNTs): pristine (Nanocyl™ CNT), acid functionalized (CNTf), or annealed treatment (CNTa). An original combination of reactive oxygen species (ROS) probes allowed the simultaneous quantifications of broad-spectrum ROS, superoxide anion (O2•−), and hydroxyl radical (•OH). All MWCNTs types induced a slight increase of broad ROS levels regardless of earlier antioxidant catalase activity. CNTf strongly stimulated the O2•− production. The •OH production was downregulated for all MWCNTs due to their scavenging capacity. The latter was quantified in a cell-free system by electron paramagnetic resonance spectroscopy (EPR). Further FCM-based assessment revealed early biological damages with a mitochondrial membrane potential collapse, followed by late cytotoxicity with chromatin decondensation. The combined evaluation by FCM analysis and cell-free techniques led to a better understanding of the impacts of MWCNTs surface treatments on the oxidative stress and related biological response. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03740357; https://hal.science/hal-03740357Test; https://hal.science/hal-03740357/documentTest; https://hal.science/hal-03740357/file/Sabido%20et%20al,%202020,%20Nanomaterials.pdfTest |
| DOI: | 10.3390/nano10020319 |
| الإتاحة: | https://doi.org/10.3390/nano10020319Test https://hal.science/hal-03740357Test https://hal.science/hal-03740357/documentTest https://hal.science/hal-03740357/file/Sabido%20et%20al,%202020,%20Nanomaterials.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.8ABF79D3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/nano10020319 |
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