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T7SS ; Tilt Series Date: 2017-06-29 Data Taken By: Debnath Ghosal Species / Specimen: Unspecified Strain: None Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 280.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Strain sent to us by Tracy Palmer, University of Dundee. Data collected and processed by Ki Woo and Debnath Ghosal. Sample Preparation: Media: TSA 500g (Oxoid TSA and TSB from Thermo Fisher. 10525943), TBS 500g (10137562)Dilute an overnight culture 1/100Incubate it for 4 hr (--> OD 2)Vitrobot ; Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-06-29-37Test Saureus_WT_06290026.mrc, Tilt Series (Pixel Size 0.39 nm), 1.9 GB Download Saureus_WT_06290026_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB Download |
