دورية أكاديمية
Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept
| العنوان: | Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept |
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| المؤلفون: | Aceituno‑Castro, Jesús, Fernández‑Muñoz, Beatriz, Navas‑García, José Manuel, Barriga‑Rivera, Alejandro, Fernández‑Lizaranzu, Isabel, Munoz‑González, Francisco Javier, Parrilla‑Giráldez, Rubén, Requena‑Lancharro, Desiree, Gil‑Gamboa, Pedro, Ramos, José Luis, Rosell-Valle, Cristina, Gómez‑González, Carmen, Martín‑López, María, Relimpio López, María Isabel, Perales‑Esteve, Manuel A., Puppo‑Moreno, Antonio, García Cózar, Francisco José, Olvera Collantes, Lucía, de los Santos‑Trigo, Silvia, Gómez, Emilia, Sánchez Pernaute, Rosario, Padillo‑Ruiz, Javier, Márquez‑Rivas, Javier, Gómez‑González, Emilio, Muñoz, Olga, Gómez Martín, Juan Carlos |
| المساهمون: | Biomedicina, Biotecnología y Salud Pública |
| المصدر: | Journal of Quantitative Spectroscopy and Radiative Transfer. Vol. 302, July 2023, 108567 |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2023 |
| المجموعة: | RODIN - Repositorio de Objetos de Docencia e Investigación de la Universidad de Cádiz |
| مصطلحات موضوعية: | COVID-19, Mueller polarimetry, Polarimetric imaging, SARS-CoV-2, Virus detection |
| الوصف: | Objective: To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. Approach: Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular stomatitis virus, and with the characteristic Spike protein of SARS-CoV-2. Samples were prepared in two biofluids (saline solution and artificial saliva), in four concentrations, and deposited as 5-µL droplets on a supporting plate. The angles of maximal degree of linear polarization (DLP) of light diffusely scattered from dry residues were determined using Mueller polarimetry from87 samples at 405 nm and 514 nm. A polarimetric camera was used for imaging several samples under 380–420 nm illumination at angles similar to those of maximal DLP. Per-pixel image analysis included quantification and combination of polarization feature descriptors in 475 samples. Main results: The angles (from sample surface) of maximal DLP were 3° for 405 nm and 6° for 514 nm. Similar viral particles that differed only in the characteristic spike protein of the SARS-CoV-2, their corresponding negative controls, fluids, and the sample holder were discerned at 10-degree and 15-degree configurations. Significance: Polarimetric imaging in the visible spectrum may help improve fast, non-contact detection and identification of viral particles, and/or other microbes such as tuberculosis, in multiple dry fluid samples simultaneously, particularly when combined with other imaging modalities. Further analysis including realistic concentrations of real SARS-CoV-2 viral particles in relevant human fluids is required. Polarimetric imaging under visible light may contribute to a fast, cost-effective screening of SARS-CoV-2 and other pathogens when combined with other imaging modalities. ; 12 páginas |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0022-4073 |
| العلاقة: | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094465-J-I00/ES/RECABLEANDO EL CEREBRO EN VISION BIONICA/; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095330-B-I00/ES/EXPERIMENTOS DE LABORATORIO, OBSERVACIONES Y MODELOS DE POLVO COMETARIO: UNA NUEVA ESTRATEGIA/; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/ES/EQC2019–006240-P; http://hdl.handle.net/10498/29550Test |
| DOI: | 10.1016/j.jqsrt.2023.108567 |
| الإتاحة: | https://doi.org/10.1016/j.jqsrt.2023.108567Test http://hdl.handle.net/10498/29550Test |
| حقوق: | Attribution-NonCommercial-NoDerivatives 4.0 Internacional ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ ; open access |
| رقم الانضمام: | edsbas.89C8309A |
| قاعدة البيانات: | BASE |
| تدمد: | 00224073 |
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| DOI: | 10.1016/j.jqsrt.2023.108567 |