التفاصيل البيبلوغرافية
| العنوان: |
Quantitative SARS-CoV-2 subgenomic RNA as a surrogate marker for viral infectivity: Comparison between culture isolation and direct sgRNA quantification |
| المؤلفون: |
Scutari, Rossana, Renica, Silvia, Cento, Valeria, Nava, Alice, Sammartino, Josè Camilla, Ferrari, Alessandro, Pani, Arianna, Merli, Marco, Fanti, Diana, Vismara, Chiara, Scaglione, Francesco, Puoti, Massimo, Bandera, Alessandra, Gori, Andrea, Piralla, Antonio, Baldanti, Fausto, Perno, Carlo Federico, Alteri, Claudia |
| المساهمون: |
Abdel-Moneim, Ahmed S., Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico |
| المصدر: |
PLOS ONE ; volume 18, issue 9, page e0291120 ; ISSN 1932-6203 |
| بيانات النشر: |
Public Library of Science (PLoS) |
| سنة النشر: |
2023 |
| المجموعة: |
PLOS Publications (via CrossRef) |
| الوصف: |
Detection of subgenomic (sg) SARS-CoV-2 RNAs are frequently used as a correlate of viral infectiousness, but few data about correlation between sg load and viable virus are available. Here, we defined concordance between culture isolation and E and N sgRNA quantification by ddPCR assays in 51 nasopharyngeal swabs collected from SARS-CoV-2 positive hospitalized patients. Among the 51 samples, 14 were SARS-CoV-2 culture-positive and 37 were negative. According to culture results, the sensitivity and specificity of E and N sgRNA assays were 100% and 100%, and 84% and 86%, respectively. ROC analysis showed that the best E and N cut-offs to predict positive culture isolation were 32 and 161 copies/mL respectively, with an AUC (95% CI) of 0.96 (0.91–1.00) and 0.96 (0.92–1.00), and a diagnostic accuracy of 88% and 92%, respectively. Even if no significant correlations were observed between sgRNA amount and clinical presentation, a higher number of moderate/severe cases and lower number of days from symptoms onset characterized patients with sgRNA equal to or higher than sgRNA cut-offs. Overall, this study suggests that SARS-CoV-2 sgRNA quantification could be helpful to estimate the replicative activity of SARS-CoV-2 and can represent a valid surrogate marker to efficiently recognize patients with active infection. The inclusion of this assay in available SARS-CoV-2 diagnostics procedure might help in optimizing fragile patients monitoring and management. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1371/journal.pone.0291120 |
| الإتاحة: |
https://doi.org/10.1371/journal.pone.0291120Test |
| حقوق: |
http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.8988BB8E |
| قاعدة البيانات: |
BASE |
