دورية أكاديمية
Gene expression profiling of myelodysplastic CD34+ hematopoietic stem cells treated in vitro with decitabine
| العنوان: | Gene expression profiling of myelodysplastic CD34+ hematopoietic stem cells treated in vitro with decitabine |
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| المؤلفون: | Giachelia, M, D'Alò, F, Fabiani, E, Saulnier, N, Di Ruscio, A, Guidi, F, Hohaus, S, Leone, G., VOSO, MARIA TERESA |
| المساهمون: | Giachelia, M, D'Alò, F, Fabiani, E, Saulnier, N, Di Ruscio, A, Guidi, F, Hohaus, S, Voso, Mt, Leone, G |
| سنة النشر: | 2011 |
| المجموعة: | Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca |
| مصطلحات موضوعية: | Adolescent, Adult, Aged, 80 and over, Antigens, CD29, CD34, Antimetabolites, Antineoplastic, Azacitidine, Cluster Analysi, DNA Methylation, Female, Gene Expression, Hematopoietic Stem Cell, Human, Male, Middle Aged, Myelodysplastic Syndrome, Oligonucleotide Array Sequence Analysi, Promoter Regions, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Young Adult, Gene Expression Profiling, Settore MED/15 - MALATTIE DEL SANGUE |
| الوصف: | Abnormal gene promoter methylation contributes to deregulate gene expression of hematopoietic progenitors in myelodysplastic syndromes (MDS). We analyzed the gene expression profile of myelodysplastic and normal CD34+ hematopoietic stem cells (HSCs) treated in vitro with decitabine. We identified a list of candidate tumor suppressor genes, expressed at low levels in MDS HSCs and induced by hypomethylating treatment only in MDS, but not in normal HSCs. Real-time RT-PCR confirmed reduced CD9 expression in MDS CD34+ and bone marrow mononuclear cells, compared to normal controls. CD9 was specifically up-regulated by decitabine treatment in myelodysplastic CD34+ cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/20869114; info:eu-repo/semantics/altIdentifier/wos/WOS:000288164600008; volume:35; issue:4; firstpage:465; lastpage:471; numberofpages:7; journal:LEUKEMIA RESEARCH; http://hdl.handle.net/2108/118378Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-79952359985 |
| DOI: | 10.1016/j.leukres.2010.07.022 |
| الإتاحة: | https://doi.org/10.1016/j.leukres.2010.07.022Test http://hdl.handle.net/2108/118378Test |
| رقم الانضمام: | edsbas.896B33E2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.leukres.2010.07.022 |
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