التفاصيل البيبلوغرافية
| العنوان: |
Absence of Angiotensin ll type 2 (AT 2 ) Receptors Increases Airway Inflammation and Responsiveness in an Allergic Mouse Model of Asthma |
| المؤلفون: |
Bhavsar, Riyakumar, Rajalingam, Sahith, Patel, Mehaben, Prasad, Praveena, Ponnoth, Dovenia S |
| المصدر: |
The FASEB Journal ; volume 33, issue S1 ; ISSN 0892-6638 1530-6860 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2019 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Angiotensin ll (Ang ll) is produced locally in the lungs and may potentiate airway responsiveness in asthmatic patients. Ang ll induces its effects by activating two GPCR subtypes: Ang II receptor type‐1 and type‐2 (AT 1 and AT 2 ). This lab has previously found that AT 2 receptor agonist plays a significant role in reducing airway inflammation and reactivity (FASEB J April 2018 32: 829.6). For the current study, we characterized the role of AT 2 receptors in airway hyperreactivity and inflammation using AT 2 receptor knockout (AT 2 KO; Hein L, Barsh et. al. Nature. 1995;377:744–747) and corresponding wild‐type (WT) mice that were divided into two experimental groups each: control (CON) and allergen sensitized‐challenged (SEN). Mice were sensitized (i.p.) on days 1, 6 with 0.2μg ovalbumin (OVA) followed by 5% OVA aerosol challenges on days 11–13. Whole body plethysmography (measuring airway responsiveness as enhanced pause, Penh) and bronchoalveolar lavage (BAL) studies were performed. Methacholine (MCh; 48mg/ml) produced significantly higher airway responsiveness in AT 2 KO SEN mice compared to WT SEN (324.7±66% in AT 2 KO SEN vs 166±56.3%, p<0.05). Total cell count analysis showed increased number of cells in AT 2 KO SEN (3.3±0.2 × 10 6 vs. 1.4±5 × 10 6 in WT SEN, p<0.001). Differential BAL cell analysis showed increased eosinophils (73±8% in AT 2 KO SEN vs. 50.11±2.84% in WT SEN, p<0.05). Macrophages were lower in AT 2 KO SEN than WT SEN (15±4% vs. 40±3%; p<0.001). These data indicate that absence of AT 2 receptors increases airway inflammation and responsiveness in our model of asthma. Support or Funding Information Institutional Research Grant Long Island University This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal . |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1096/fasebj.2019.33.1_supplement.515.15 |
| الإتاحة: |
https://doi.org/10.1096/fasebj.2019.33.1_supplement.515.15Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.89543D2D |
| قاعدة البيانات: |
BASE |
