التفاصيل البيبلوغرافية
| العنوان: |
C57BL/6 and Congenic Interleukin-10-Deficient Mice Can Serve as Models of Campylobacter jejuni Colonization and Enteritis |
| المؤلفون: |
Mansfield, L. S., Bell, J. A., Wilson, D. L., Murphy, A. J., Elsheikha, H. M., Rathinam, V. A. K., Fierro, B. R., Linz, J. E., Young, V. B. |
| المصدر: |
Infection and Immunity ; volume 75, issue 3, page 1099-1115 ; ISSN 0019-9567 1098-5522 |
| بيانات النشر: |
American Society for Microbiology |
| سنة النشر: |
2007 |
| الوصف: |
Campylobacter jejuni is a globally distributed cause of human food-borne enteritis and has been linked to chronic joint and neurological diseases. We hypothesized that C. jejuni 11168 colonizes the gastrointestinal tract of both C57BL/6 mice and congenic C57BL/6 interleukin-10-deficient (IL-10 −/− ) mice and that C57BL/6 IL-10 −/− mice experience C. jejuni 11168-mediated clinical signs and pathology. Individually housed mice were challenged orally with C. jejuni 11168, and the course of infection was monitored by clinical examination, bacterial culture, C. jejuni -specific PCR, gross pathology, histopathology, immunohistochemistry, and anti- C. jejuni -specific serology. Ceca of C. jejuni 11168-infected mice were colonized at high rates: ceca of 50/50 wild-type mice and 168/170 IL-10 −/− mice were colonized. In a range from 2 to 35 days after infection with C. jejuni 11168, C57BL/6 IL-10 −/− mice developed severe typhlocolitis best evaluated at the ileocecocolic junction. Rates of colonization and enteritis did not differ between male and female mice. A dose-response experiment showed that as little as 10 6 CFU produced significant disease and pathological lesions similar to responses seen in humans. Immunohistochemical staining demonstrated C. jejuni antigens within gastrointestinal tissues of infected mice. Significant anti- C. jejuni plasma immunoglobulin levels developed by day 28 after infection in both wild-type and IL-10-deficient animals; antibodies were predominantly T-helper-cell 1 (Th1)-associated subtypes. These results indicate that the colonization of the mouse gastrointestinal tract by C. jejuni 11168 is necessary but not sufficient for the development of enteritis and that C57BL/6 IL-10 −/− mice can serve as models for the study of C. jejuni enteritis in humans. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1128/iai.00833-06 |
| DOI: |
10.1128/IAI.00833-06 |
| الإتاحة: |
https://doi.org/10.1128/iai.00833-06Test |
| حقوق: |
https://journals.asm.org/non-commercial-tdm-licenseTest |
| رقم الانضمام: |
edsbas.88B23331 |
| قاعدة البيانات: |
BASE |
