التفاصيل البيبلوغرافية
| العنوان: |
Natural killer cell and T-cell subset distributions and activation influence susceptibility to perinatal HIV-1 infection |
| المؤلفون: |
Wamalwa, Dalton, Maleche-Obimbo, Elizabeth, Bosire, Rose, Lejarcegui, Nicholas, Itaya, Grace, Kunwar, Pratima, Gasper, Melanie A |
| سنة النشر: |
2014 |
| المجموعة: |
University of Nairobi Digital Repository |
| الوصف: |
OBJECTIVE: To determine neonatal immunologic factors that correlate with mother-to-child-transmission of HIV-1. DESIGN: This case-control study compared cord blood natural killer (NK) and T-cell populations of HIV-1 exposed infants who subsequently acquired infection by 1 month (cases) to those who remained uninfected by 1 year of life (controls). Control specimens were selected by proportional match on maternal viral load. METHODS: Cryopreserved cord blood mononuclear cells (CBMCs) were thawed and stained for multiparameter flow cytometry to detect NK and T-cell subsets and activation status. CBMCs were also used in a viral suppression assay to evaluate NK cell inhibition of HIV-1 replication in autologous CD4 T cells. RESULTS: Cord blood from cases contained a skewed NK cell repertoire characterized by an increased proportion of CD16CD56 NK cells. In addition, cases displayed less-activated CD16CD56 NK cells and CD8 T cells, based on HLA-DRCD38 costaining. NK cell suppression of HIV-1 replication ex vivo correlated with the proportion of acutely activated CD68CD16CD56 NK cells. Finally, we detected a higher proportion of CD27CD45RA effector memory CD4 and CD8 T cells in cord blood from cases compared with controls. CONCLUSION: When controlled for maternal viral load, cord blood from infants who acquired HIV-1 had a higher proportion of CD16CD56 NK cells, lower NK cell activation and higher levels of mature T cells (potential HIV-1 targets) than control infants who remained uninfected. Our data provide evidence that infant HIV-1 acquisition may be influenced by both innate and adaptive immune cell phenotypes and activation status. ; a Department of Psychiatry, University of Nairobi, ; bDepartment of Mental Health, School of Medicine, Moi University, Eldoret, Kenya |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| العلاقة: |
Gasper, Melanie A., Pratima Kunwar, Grace Itaya, Nicholas Lejarcegui, Rose Bosire, Elizabeth Maleche-Obimbo, Dalton Wamalwa et al. "Natural killer cell and T-cell subset distributions and activation influence susceptibility to perinatal HIV-1 infection." AIDS 28, no. 8 (2014): 1115-1124.; http://hdl.handle.net/11295/66270Test |
| الإتاحة: |
http://hdl.handle.net/11295/66270Test |
| رقم الانضمام: |
edsbas.87F7B2EE |
| قاعدة البيانات: |
BASE |
