دورية أكاديمية
Effects of ketamine and thiopental on ischemia reoxygenation-induced LDH leakage and amino acid release from rat striatal slices
| العنوان: | Effects of ketamine and thiopental on ischemia reoxygenation-induced LDH leakage and amino acid release from rat striatal slices |
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| المساهمون: | Uludağ Üniversitesi/Tıp Fakültesi/Anestezi ve Reanimasyon Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji ve Klinik Farmakoloji Anabilim Dalı., Başağan Moğol, Elif, Büyükuysal, Rifat Levent, Korfalı, Gülsen, AAH-1657-2021, 23982134100, 6602686612, 6701462594 |
| بيانات النشر: | Lippincott Williams & Wilkins |
| سنة النشر: | 2005 |
| المجموعة: | Açık Erişim@BUU (Bursa Uludağ Üniversitesi) |
| مصطلحات موضوعية: | Ischemia/reoxygenation, LDH, Amino acids, Ketamine, Thiopental, Glutamate release, Hippocampal slices, Cerebral-ıschemia, In-vitroglucose, Deprivation, Neuronal death, Brain ıschemia, Cerebrocortical slices, Lipid-peroxidation, NA+/CA2+ Channel, Anesthesiology, Neurosciences & neurology, Surgery, Anesthetics, dissociative, intravenous, Animals, Brain ischemia, Female, L-lactate dehydrogenase, Male, Proteins, Rats, wistar, Reperfusion |
| الوصف: | Bu çalışma, 6-9 Nisan 2002 tarihleri arasında Fransa'da düzenlenen 10th Annual Meeting of the European-Society-of-Anaesthesiologists'de bildiri olarak sunulmuştur. ; Increased release of glutamate is thought to contribute to ischemia-induced neuronal damage. Since general anesthetics such as thiopental and ketamine are thought to provide some degree of cerebral protection, this study was intended to 1) compare the effectiveness of ketamine and thiopental on ischemia-induced tissue damage; and, if so, 2) determine whether attenuation of the increased amino acid release is the sole mechanism for the protective effects demonstrated. Striatal slices prepared from Wistar Albino rats were incubated in an ischemic medium for 1 hour followed by 5 hours in a reoxygenation (REO) medium. Ketamine and thiopental were added medium during ischemia and/or REO periods, and the medium was collected at the end of each incubation period for measurement of amino acid release and lactate dehydrogenase (LDH) leakage. Ischemia significantly increased amino acid release without altering LDH leakage. Ischemia-induced increments in glutamate and aspartic acid releases returned to control levels during REO, but LDH leakage increased (P < 0.001) during this period. Although ketamine (100 muM) and thiopental (100 mu\M) failed to decrease ischermia-induced excitatory amino acid release, they protected the slices against REO-induced LDH leakage. Ketamine, but not thiopental, was effective even if added after ischemia (P < 0.05). These results indicate that ketamine and thiopental protect the slices against REO-induced LDH leakage. However, mechanisms other than attenuation of the enhanced glutamate release might be responsible for their protective effects. |
| نوع الوثيقة: | article in journal/newspaper report |
| اللغة: | English |
| تدمد: | 0898-4921 |
| العلاقة: | Makale - Uluslararası Hakemli Dergi; Journal of Neurosurgical Anestesiology; Başağan-Moğol, E. vd. (2005). "Effects of ketamine and thiopental on ischemia reoxygenation-induced LDH leakage and amino acid release from rat striatal slices". Journal of Neurosurgical Anesthesiology, 17(1), 20-26.; http://hdl.handle.net/11452/21636Test; 000226154900006; 2-s2.0-11244309314; 20; 26; 17 |
| الإتاحة: | http://hdl.handle.net/11452/21636Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.87B95C91 |
| قاعدة البيانات: | BASE |
| تدمد: | 08984921 |
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