دورية أكاديمية
Towards a structurally resolved human protein interaction network
| العنوان: | Towards a structurally resolved human protein interaction network |
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| المؤلفون: | Burke, David F., Bryant, Patrick, Barrio-Hernandez, Inigo, Memon, Danish, Pozzati, Gabriele, Shenoy, Aditi, Zhu, Wensi, Dunham, Alistair S., Albanese, Pascal, Keller, Andrew, Scheltema, Richard A., Bruce, James E., Leitner, Alexander, Kundrotas, Petras, Beltrao, Pedro, id_orcid:0 000-0002-2724-7703, Elofsson, Arne |
| المصدر: | Nature Structural & Molecular Biology, 30 (2) |
| بيانات النشر: | Nature |
| سنة النشر: | 2023 |
| المجموعة: | ETH Zürich Research Collection |
| الوصف: | Cellular functions are governed by molecular machines that assemble through protein-protein interactions. Their atomic details are critical to studying their molecular mechanisms. However, fewer than 5% of hundreds of thousands of human protein interactions have been structurally characterized. Here we test the potential and limitations of recent progress in deep-learning methods using AlphaFold2 to predict structures for 65,484 human protein interactions. We show that experiments can orthogonally confirm higher-confidence models. We identify 3,137 high-confidence models, of which 1,371 have no homology to a known structure. We identify interface residues harboring disease mutations, suggesting potential mechanisms for pathogenic variants. Groups of interface phosphorylation sites show patterns of co-regulation across conditions, suggestive of coordinated tuning of multiple protein interactions as signaling responses. Finally, we provide examples of how the predicted binary complexes can be used to build larger assemblies helping to expand our understanding of human cell biology. ; ISSN:1545-9993 ; ISSN:1545-9985 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/000928325000001; info:eu-repo/grantAgreement/EC/H2020/823839; http://hdl.handle.net/20.500.11850/600784Test |
| DOI: | 10.3929/ethz-b-000600784 |
| الإتاحة: | https://doi.org/20.500.11850/600784Test https://doi.org/10.3929/ethz-b-000600784Test https://doi.org/10.1038/s41594-022-00910-8Test https://hdl.handle.net/20.500.11850/600784Test |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0Test/ ; Creative Commons Attribution 4.0 International |
| رقم الانضمام: | edsbas.87896CF9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3929/ethz-b-000600784 |
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