دورية أكاديمية
Phylogeny, Multiple Antibiotic Resistance Index and BiofilmCharacterization of Mannheimia species Isolated from ClinicalCases of Pneumonia in Small Ruminants
| العنوان: | Phylogeny, Multiple Antibiotic Resistance Index and BiofilmCharacterization of Mannheimia species Isolated from ClinicalCases of Pneumonia in Small Ruminants |
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| المؤلفون: | Thatavarthi, Swaroop N S R K M, Sivarama , Krishna G, Supriya, A R, Anand , Kumar P |
| المصدر: | Indian Journal of Veterinary Sciences and Biotechnology; Vol. 20 No. 2 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Mar-Apr) 2024; 7-13 ; 2395-1176 ; 2394-0247 ; 10.48165/ |
| بيانات النشر: | Indian Journal of Veterinary Sciences & Biotechnology |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biofilms, Mannheimia, MAR-index, 16S rRNA, Phylogeny |
| الوصف: | Mannheimia is a Gram-negative, bipolar organism that is frequently involved in respiratory infections of bovines and small ruminants. In the present study, a total of 92 nasal swab samples were examined from enzootic pneumonia cases of sheep and goat, out of which 30 Mannheimia isolates have been identified. The 16S rRNA sequence analysis of the isolate revealed a close genetic distance between the Mannheimia species from different geographical regions. However, it formed a separate clade in the phylogenetic tree and the isolate is closely related to the M. hemolytica and M. caviae species. Three methods (Standard Tube method, Congo Red method and Microtiter Plate assay) were evaluated for identifying the biofilm forming Mannheimia isolates. Among them, Microtiter plate assay was the best method for quantification of biofilm production, by which 29 out of 30 isolates were identified as biofilm producers. In antibiogram study, the Mannheimia isolates exhibited resistance towards ampicillins, gentamicin, co-trimoxazole, tetracyclines and amoxicillin. All 30 isolates developed a multiple antibiotic resistance index (MAR) of more than 0.2. This indicates that there is a potential threat of antimicrobial resistance (AMR) transmission through food animals. In conclusion our study provides insights into the genetic relation between Mannheimia from India with other Mannheimia species from a different geographical region, and their capabilities of biofilm production. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://acspublisher.com/journals/index.php/ijvsbt/article/view/11617/12244Test; https://acspublisher.com/journals/index.php/ijvsbt/article/view/11617Test |
| DOI: | 10.48165/ijvsbt.20.2.02 |
| الإتاحة: | https://doi.org/10.48165/ijvsbt.20.2.02Test https://acspublisher.com/journals/index.php/ijvsbt/article/view/11617Test |
| حقوق: | Copyright (c) 2024 Indian Journal of Veterinary Sciences & Biotechnology ; https://creativecommons.org/licenses/by-nc-nd/4.0Test |
| رقم الانضمام: | edsbas.862335EF |
| قاعدة البيانات: | BASE |
| DOI: | 10.48165/ijvsbt.20.2.02 |
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