تقرير
Natural variation in salt-induced changes in root:shoot ratio reveals SR3G as a negative regulator of root suberization and salt resilience in Arabidopsis
| العنوان: | Natural variation in salt-induced changes in root:shoot ratio reveals SR3G as a negative regulator of root suberization and salt resilience in Arabidopsis |
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| المؤلفون: | Ishka, Maryam Rahmati, Sussman, Hayley, Hu, Yunfei, Alqahtani, Mashael Daghash Saeed, Craft, Eric, Sicat, Ronell Barrera, Wang, Minmin, Yu, Li'ang, Ait-Haddou, Rachid, Li, Bo, Drakakaki, Georgia, Nelson, Andrew, Pineros, Miguel, Korte, Arthur, Jaremko, Lukasz, Testerink, Christa, Tester, Mark A., Julkowska, Magdalena |
| المساهمون: | Bioscience, Bioscience Program, Biological, Environmental Sciences and Engineering, Biological and Environmental Science and Engineering (BESE) Division, Visualization, Visual Computing Center (VCC), Computer, Electrical and Mathematical Sciences and Engineering, Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division, Plant Science, Center for Desert Agriculture, Water Desalination & Reuse Center, Water Desalination and Reuse Research Center (WDRC), Visualization Laboratory, KAUST Visualization Laboratory (KVL), Boyce Thompson Institute, Ithaca, NY, USA, Lanzhou University, Lanzhou, China, USDA-ARS, Ithaca, NY, USA, UC Davis, Davis, CA, USA, Julius-von-Sachs-Institute & Center for Computational and Theoretical Biology, JMU, Wuerzburg, Germany, Wageningen Research and University, Wageningen, The Netherlands |
| بيانات النشر: | Cold Spring Harbor Laboratory |
| سنة النشر: | 2024 |
| المجموعة: | King Abdullah University of Science and Technology: KAUST Repository |
| الوصف: | Soil salinity is one of the major threats to agricultural productivity worldwide. Salt stress exposure alters root and shoot growth rates, thereby affecting overall plant performance. While past studies have extensively documented the effect of salt stress on root elongation and shoot development separately, here we take an innovative approach by examining the coordination of root and shoot growth under salt stress conditions. Utilizing a newly developed tool for quantifying the root:shoot ratio in agar-grown Arabidopsis seedlings, we found that salt stress results in a loss of coordination between root and shoot growth rates. We identify a specific gene cluster encoding domain-of-unknown-function 247 (DUF247), and characterize one of these genes as Salt Root:shoot Ratio Regulator Gene (SR3G). Further analysis elucidates the role of SR3G as a negative regulator of salt stress tolerance, revealing its function in regulating shoot growth, root suberization, and sodium accumulation. We further characterize that SR3G expression is modulated by WRKY75 transcription factor, known as a positive regulator of salt stress tolerance. Finally, we show that the salt stress sensitivity of wrky75 mutant is completely diminished when it is combined with sr3g mutation. Together, our results demonstrate that utilizing root:shoot ratio as an architectural feature leads to the discovery of new stress resilience gene. The study's innovative approach and findings not only contribute to our understanding of plant stress tolerance mechanisms but also open new avenues for genetic and agronomic strategies to enhance crop environmental resilience. ; The authors would like to thank BTI and KAUST Greenhouse Teams for their care of the plants. The authors would like to acknowledge support from the NSF-IOS #2023310 (ADLN) and NSF-IOS #2102120 (ADLN). The majority of funding for this work was generously provided from KAUST baseline funding awarded to Mark Tester, and BTI’s startup funds awarded to Magdalena Julkowska. |
| نوع الوثيقة: | report |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/10754/698041Test |
| DOI: | 10.1101/2024.04.09.588564 |
| الإتاحة: | https://doi.org/10.1101/2024.04.09.588564Test http://hdl.handle.net/10754/698041Test |
| حقوق: | This is a preprint version of a paper and has not been peer reviewed. Archived with thanks to Cold Spring Harbor Laboratory. |
| رقم الانضمام: | edsbas.85DB868F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2024.04.09.588564 |
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