التفاصيل البيبلوغرافية
| العنوان: |
Presentation_1_Human Cytomegalovirus Infection Changes the Pattern of Surface Markers of Small Extracellular Vesicles Isolated From First Trimester Placental Long-Term Histocultures.pdf |
| المؤلفون: |
Mathilde Bergamelli (11416334), Hélène Martin (232730), Mélinda Bénard (769549), Jérôme Ausseil (92331), Jean-Michel Mansuy (11416337), Ilse Hurbain (168655), Maïlys Mouysset (11416340), Marion Groussolles (11416343), Géraldine Cartron (399558), Yann Tanguy le Gac (11416346), Nathalie Moinard (11416349), Elsa Suberbielle (196793), Jacques Izopet (114143), Charlotte Tscherning (11416352), Graça Raposo (205160), Daniel Gonzalez-Dunia (61852), Gisela D’Angelo (11416355), Cécile E. Malnou (11416358) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Cell Biology, Marine Biology, Cell Development, Proliferation and Death, Cell Metabolism, Cell Neurochemistry, Cellular Interactions (incl. Adhesion, Matrix, Cell Wall), early placenta, extracellular vesicles, congenital infection, human cytomegalovirus, placental histoculture |
| الوصف: |
Extracellular vesicles (EVs) have increasingly been recognized as key players in a wide variety of physiological and pathological contexts, including during pregnancy. Notably, EVs appear both as possible biomarkers and as mediators involved in the communication of the placenta with the maternal and fetal sides. A better understanding of the physiological and pathological roles of EVs strongly depends on the development of adequate and reliable study models, specifically at the beginning of pregnancy where many adverse pregnancy outcomes have their origin. In this study, we describe the isolation of small EVs from a histoculture model of first trimester placental explants in normal conditions as well as upon infection by human cytomegalovirus. Using bead-based multiplex cytometry and electron microscopy combined with biochemical approaches, we characterized these small EVs and defined their associated markers and ultrastructure. We observed that infection led to changes in the expression level of several surface markers, without affecting the secretion and integrity of small EVs. Our findings lay the foundation for studying the functional role of EVs during early pregnancy, along with the identification of new predictive biomarkers for the severity and outcome of this congenital infection, which are still sorely lacking. |
| نوع الوثيقة: |
conference object |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/presentation/Presentation_1_Human_Cytomegalovirus_Infection_Changes_the_Pattern_of_Surface_Markers_of_Small_Extracellular_Vesicles_Isolated_From_First_Trimester_Placental_Long-Term_Histocultures_pdf/16600307Test |
| DOI: |
10.3389/fcell.2021.689122.s001 |
| الإتاحة: |
https://doi.org/10.3389/fcell.2021.689122.s001Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.85BB7F9B |
| قاعدة البيانات: |
BASE |
