دورية أكاديمية
Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B
| العنوان: | Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B |
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| المؤلفون: | Hegarat, Nadia, Crncec, Adrijana, Suarez Peredoa Rodriguez, Maria F., Iturra, Fabio Echegaray, Gu, Yan, Busby, Oliver, Lang, Paul F., Barr, Alexis R., Bakal, Chris, Kanemaki, Masato T., Lamond, Angus I., Novak, Bela, Ly, Tony, Hochegger, Helfrid |
| المصدر: | Hegarat , N , Crncec , A , Suarez Peredoa Rodriguez , M F , Iturra , F E , Gu , Y , Busby , O , Lang , P F , Barr , A R , Bakal , C , Kanemaki , M T , Lamond , A I , Novak , B , Ly , T & Hochegger , H 2020 , ' Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B ' , EMBO Journal , vol. 39 , no. 11 , e104419 . https://doi.org/10.15252/embj.2020104419Test |
| سنة النشر: | 2020 |
| المجموعة: | Discovery - University of Dundee Online Publications |
| مصطلحات موضوعية: | Cdk1, Cyclin, Greatwall, MASTL, PP2A, /dk/atira/pure/subjectarea/asjc/1300/1300, name=General Biochemistry,Genetics and Molecular Biology, /dk/atira/pure/subjectarea/asjc/2400/2400, name=General Immunology and Microbiology, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/2800/2800, name=General Neuroscience |
| الوصف: | Two mitotic cyclin types, cyclin A and B, exist in higher eukaryotes, but their specialised functions in mitosis are incompletely understood. Using degron tags for rapid inducible protein removal, we analyse how acute depletion of these proteins affects mitosis. Loss of cyclin A in G2-phase prevents mitotic entry. Cells lacking cyclin B can enter mitosis and phosphorylate most mitotic proteins, because of parallel PP2A:B55 phosphatase inactivation by Greatwall kinase. The final barrier to mitotic establishment corresponds to nuclear envelope breakdown, which requires a decisive shift in the balance of cyclin-dependent kinase Cdk1 and PP2A:B55 activity. Beyond this point, cyclin B/Cdk1 is essential for phosphorylation of a distinct subset of mitotic Cdk1 substrates that are essential to complete cell division. Our results identify how cyclin A, cyclin B and Greatwall kinase coordinate mitotic progression by increasing levels of Cdk1-dependent substrate phosphorylation. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://discovery.dundee.ac.uk/en/publications/d292c1db-0e3d-45d6-a89a-f16b4f93df15Test |
| DOI: | 10.15252/embj.2020104419 |
| الإتاحة: | https://doi.org/10.15252/embj.2020104419Test https://discovery.dundee.ac.uk/en/publications/d292c1db-0e3d-45d6-a89a-f16b4f93df15Test https://discovery.dundee.ac.uk/ws/files/49074165/embj.2020104419.pdfTest http://www.scopus.com/inward/record.url?scp=85083961569&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.853E5D85 |
| قاعدة البيانات: | BASE |
| DOI: | 10.15252/embj.2020104419 |
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