دورية أكاديمية
A Two-pronged Binding Mechanism of IgG to the Neonatal Fc Receptor Controls Complex Stability and IgG Serum Half-life
| العنوان: | A Two-pronged Binding Mechanism of IgG to the Neonatal Fc Receptor Controls Complex Stability and IgG Serum Half-life |
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| المؤلفون: | Jensen, Pernille Foged, Schoch, Angela, Larraillet, Vincent, Hilger, Maximiliane, Schlothauer, Tilman, Emrich, Thomas, Rand, Kasper Dyrberg |
| المصدر: | Jensen , P F , Schoch , A , Larraillet , V , Hilger , M , Schlothauer , T , Emrich , T & Rand , K D 2017 , ' A Two-pronged Binding Mechanism of IgG to the Neonatal Fc Receptor Controls Complex Stability and IgG Serum Half-life ' , Molecular and Cellular Proteomics , vol. 16 , no. 3 , pp. 451-456 . https://doi.org/10.1074/mcp.M116.064675Test |
| سنة النشر: | 2017 |
| المجموعة: | University of Copenhagen: Research / Forskning ved Københavns Universitet |
| مصطلحات موضوعية: | Antibodies, Monoclonal/chemistry, Binding Sites, Deuterium Exchange Measurement/methods, Half-Life, Histocompatibility Antigens Class I/metabolism, Humans, Hydrogen-Ion Concentration, Immunoglobulin G/chemistry, Mass Spectrometry/methods, Models, Molecular, Protein Binding, Protein Stability, Receptors, Fc/metabolism |
| الوصف: | The success of recombinant monoclonal immunoglobulins (IgG) is rooted in their ability to target distinct antigens with high affinity combined with an extraordinarily long serum half-life, typically around 3 weeks. The pharmacokinetics of IgGs is intimately linked to the recycling mechanism of the neonatal Fc receptor (FcRn). For long serum half-life of therapeutic IgGs, the highly pH-dependent interaction with FcRn needs to be balanced to allow efficient FcRn binding and release at slightly acidic pH and physiological pH, respectively. Some IgGs, like the antibody briakinumab has an unusually short half-life of ∼8 days. Here we dissect the molecular origins of excessive FcRn binding in therapeutic IgGs using a combination of hydrogen/deuterium exchange mass spectrometry and FcRn affinity chromatography. We provide experimental evidence for a two-pronged IgG-FcRn binding mechanism involving direct FcRn interactions with both the Fc region and the Fab regions of briakinumab, and correlate the occurrence of excessive FcRn binding to an unusually strong Fab-FcRn interaction. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1074/mcp.M116.064675 |
| الإتاحة: | https://doi.org/10.1074/mcp.M116.064675Test https://curis.ku.dk/portal/da/publications/a-twopronged-binding-mechanism-of-igg-to-the-neonatal-fc-receptor-controls-complex-stability-and-igg-serum-halflifeTest(17c9172d-f7ef-40e1-8929-838b76eb8e52).html https://curis.ku.dk/ws/files/196136206/451.full.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.83C43D83 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1074/mcp.M116.064675 |
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