دورية أكاديمية
Formation of non-base-pairing DNA microgels using directed phase transition of amphiphilic monomers
| العنوان: | Formation of non-base-pairing DNA microgels using directed phase transition of amphiphilic monomers |
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| المؤلفون: | Lee, Chanseok, Do, Sungho, Lee, Jae Young, Kim, Minju, Kim, Sang Moon, Shin, Yongdae, Kim, Do-Nyun |
| المساهمون: | National Convergence Research of Scientific Challenges, National Research Foundation of Korea, Ministry of Science and ICT, National Supercomputing Center, Ministry of Education |
| المصدر: | Nucleic Acids Research ; volume 50, issue 7, page 4187-4196 ; ISSN 0305-1048 1362-4962 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2022 |
| الوصف: | Programmability of DNA sequences enables the formation of synthetic DNA nanostructures and their macromolecular assemblies such as DNA hydrogels. The base pair-level interaction of DNA is a foundational and powerful mechanism to build DNA structures at the nanoscale; however, its temperature sensitivity and weak interaction force remain a barrier for the facile and scalable assembly of DNA structures toward higher-order structures. We conducted this study to provide an alternative, non-base-pairing approach to connect nanoscale DNA units to yield micrometer-sized gels based on the sequential phase transition of amphiphilic unit structures. Strong electrostatic interactions between DNA nanostructures and polyelectrolyte spermines led to the formation of giant phase-separated aggregates of monomer units. Gelation could be initiated by the addition of NaCl, which weakened the electrostatic DNA-spermine interaction while attractive interactions between cholesterols created stable networks by crosslinking DNA monomers. In contrast to the conventional DNA gelation techniques, our system used solid aggregates as a precursor for DNA microgels. Therefore, in situ gelation could be achieved by depositing aggregates on the desired substrate and subsequently initiating a phase transition. Our approach can expand the utility and functionality of DNA hydrogels by using more complex nucleic acid assemblies as unit structures and combining the technique with top-down microfabrication methods. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/nar/gkac232 |
| الإتاحة: | https://doi.org/10.1093/nar/gkac232Test https://academic.oup.com/nar/article-pdf/50/7/4187/43402446/gkac232.pdfTest |
| حقوق: | https://creativecommons.org/licenses/by-nc/4.0Test/ |
| رقم الانضمام: | edsbas.8309E9F9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/gkac232 |
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