دورية أكاديمية
The DEAD-Box RNA Helicase Ded1 Is Associated with Translating Ribosomes
| العنوان: | The DEAD-Box RNA Helicase Ded1 Is Associated with Translating Ribosomes |
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| المؤلفون: | Yeter-Alat, Hilal, Belgareh-Touzé, Naïma, Huvelle, Emmeline, Banroques, Josette, Tanner, N, Kyle |
| المساهمون: | Expression Génétique Microbienne (EGM (UMR_8261 / FRE_3630)), Institut de biologie physico-chimique (IBPC (FR_550)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Université Paris Sciences et Lettres (PSL), Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes (LBMCE), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), ANR-11-LABX-0011,DYNAMO,Dynamique des membranes transductrices d'énergie : biogénèse et organisation supramoléculaire.(2011) |
| المصدر: | ISSN: 2073-4425 ; Genes ; https://hal.science/hal-04202438Test ; Genes, 2023, 14 (8), pp.1566. ⟨10.3390/genes14081566⟩. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Ded1/DDX3, UV-A, ATPase, DEAD-box, qtPAR-CLIP, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] |
| الوصف: | International audience ; DEAD-box RNA helicases are ATP-dependent RNA binding proteins and RNA-dependent ATPases that possess weak, nonprocessive unwinding activity in vitro, but they can form long-lived complexes on RNAs when the ATPase activity is inhibited. Ded1 is a yeast DEAD-box protein, the functional ortholog of mammalian DDX3, that is considered important for the scanning efficiency of the 48S pre-initiation complex ribosomes to the AUG start codon. We used a modified PAR-CLIP technique, which we call quicktime PAR-CLIP (qtPAR-CLIP), to crosslink Ded1 to 4-thiouridineincorporated RNAs in vivo using UV light centered at 365 nm. The irradiation conditions are largely benign to the yeast cells and to Ded1, and we are able to obtain a high efficiency of crosslinking under physiological conditions. We find that Ded1 forms crosslinks on the open reading frames of many different mRNAs, but it forms the most extensive interactions on relatively few mRNAs, and particularly on mRNAs encoding certain ribosomal proteins and translation factors. Under glucosedepletion conditions, the crosslinking pattern shifts to mRNAs encoding metabolic and stress-related proteins, which reflects the altered translation. These data are consistent with Ded1 functioning in the regulation of translation elongation, perhaps by pausing or stabilizing the ribosomes through its ATP-dependent binding. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-04202438; https://hal.science/hal-04202438Test; https://hal.science/hal-04202438/documentTest; https://hal.science/hal-04202438/file/genes-14-01566.pdfTest |
| DOI: | 10.3390/genes14081566 |
| الإتاحة: | https://doi.org/10.3390/genes14081566Test https://hal.science/hal-04202438Test https://hal.science/hal-04202438/documentTest https://hal.science/hal-04202438/file/genes-14-01566.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.82FDDE0E |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/genes14081566 |
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